Molecularly imprinted fluorescence sensor chip for lactate measurement.

Area of Science:

• Biomedical Engineering
• Materials Science
• Analytical Chemistry

Background:

• Lactate measurements are crucial for assessing bodily functions and athletic performance.
• Existing enzyme-based lactate sensors have limitations, necessitating innovative detection methods.
• Molecularly imprinted polymers (MIPs) offer high selectivity for target analyte detection.

Purpose of the Study:

• To develop a novel molecularly imprinted fluorescence biochip for sensitive and selective lactate detection.
• To overcome the limitations of traditional enzyme-based sensors and liquid-based detection platforms.
• To create a real-time, solid-phase sensing platform for lactate analysis.

Main Methods:

• Fabrication of a biosensor chip integrating ZnO quantum dots (QDs) with MIPs.
• Formation of lactate-selective MIPs using 3-aminopropyltriethoxysilane (APTES) and 5-indolyl boronic acid monomers.
• Development of a solid-phase sensing platform utilizing UV excitation and a portable light detector for real-time measurements.

Main Results:

• The developed sensor demonstrated high sensitivity (0.0217 mmol L⁻¹) for lactate detection within the 0-30 mmol L⁻¹ range.
• A high correlation coefficient (0.97) was achieved in phosphate-buffered saline (PBS) solution.
• The sensor exhibited high sensitivity and selectivity, indicating potential for sweat analysis.

Conclusions:

• The molecularly imprinted fluorescence biochip represents an innovative approach to lactate measurement.
• ZnO QDs combined with MIPs provide a robust platform for sensitive and selective lactate detection.
• The developed solid-phase sensor is suitable for real-time lactate monitoring in biological samples like sweat.