Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

6.4K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Rise of Trapped Ion Mobility Spectrometry: Principles, Applications, and Recent Developments.

Mass spectrometry reviews·2026
Same author

Legends of Ion Mobility Spectrometry - Martin F. Jarrold.

Journal of the American Society for Mass Spectrometry·2026
Same author

Postmortem redistribution and quantitative analysis of bromazolam, etizolam, flualprazolam, and flubromazolam.

Journal of analytical toxicology·2026
Same author

Legends of Ion Mobility Spectrometry - Glenn E. Spangler.

Journal of the American Society for Mass Spectrometry·2026
Same author

An enduring threat: NPS benzodiazepines in forensic and clinical toxicology from 2021 to 2025.

Journal of analytical toxicology·2026
Same author

Structural Landscape of Syanodin I Lasso Peptide.

Biochemistry·2026

Related Experiment Video

Updated: Jun 6, 2025

Author Spotlight: An Efficient Methodology to Confidently Differentiate and Characterize Fentanyl Analogs
10:13

Author Spotlight: An Efficient Methodology to Confidently Differentiate and Characterize Fentanyl Analogs

Published on: November 8, 2024

2.0K

Fentanyl Analog Screening using LC-TIMS-TOF MS/MS.

Andrew R Forero1, Lilian Valadares Tose1, Matthew Willetts2

  • 1Department of Chemistry and Biochemistry, Florida International University.

Journal of Visualized Experiments : Jove
|November 25, 2024
PubMed
Summary

A new high-throughput analytical protocol screens hundreds of fentanyl analogs using liquid chromatography, ion mobility, and mass spectrometry. This method aids in identifying potent fentanyl analogs contributing to overdose deaths.

More Related Videos

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
10:17

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

Published on: April 23, 2019

9.6K
Identifying Per- and Polyfluorinated Chemical Species with a Combined Targeted and Non-Targeted-Screening High-Resolution Mass Spectrometry Workflow
09:04

Identifying Per- and Polyfluorinated Chemical Species with a Combined Targeted and Non-Targeted-Screening High-Resolution Mass Spectrometry Workflow

Published on: April 18, 2019

12.4K

Related Experiment Videos

Last Updated: Jun 6, 2025

Author Spotlight: An Efficient Methodology to Confidently Differentiate and Characterize Fentanyl Analogs
10:13

Author Spotlight: An Efficient Methodology to Confidently Differentiate and Characterize Fentanyl Analogs

Published on: November 8, 2024

2.0K
High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
10:17

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

Published on: April 23, 2019

9.6K
Identifying Per- and Polyfluorinated Chemical Species with a Combined Targeted and Non-Targeted-Screening High-Resolution Mass Spectrometry Workflow
09:04

Identifying Per- and Polyfluorinated Chemical Species with a Combined Targeted and Non-Targeted-Screening High-Resolution Mass Spectrometry Workflow

Published on: April 18, 2019

12.4K

Area of Science:

  • Forensic Chemistry
  • Analytical Chemistry
  • Toxicology

Background:

  • Fentanyl and its analogs are a major cause of overdose deaths in the United States.
  • Illicitly manufactured fentanyl's extreme potency contributes to most recent overdose cases.
  • There is a growing need for rapid and comprehensive analytical methods to detect these substances.

Purpose of the Study:

  • To develop a high-throughput analytical protocol for screening fentanyl analogs.
  • To enable the separation and identification of numerous fentanyl analogs from complex samples.
  • To provide a reliable method for forensic and toxicological analysis.

Main Methods:

  • Utilized a combination of liquid chromatography (LC), trapped ion mobility spectrometry (TIMS), and tandem mass spectrometry (MS/MS).
  • Employed data-dependent acquisition (DDA) and data-independent acquisition (DIA) strategies.
  • Incorporated parallel accumulation in the mobility trap followed by sequential collision-induced dissociation (CID) fragmentation.

Main Results:

  • Successfully separated and assigned hundreds of fentanyl analogs in a single sample and scan.
  • Achieved confident analog identification based on retention time, ion mobility, and MS/MS fragmentation patterns.
  • Demonstrated the protocol's effectiveness for high-throughput screening.

Conclusions:

  • The developed LC-TIMS-MS/MS protocol offers a powerful tool for detecting and identifying fentanyl analogs.
  • This method can significantly aid in monitoring the evolving landscape of synthetic opioids.
  • The protocol supports forensic investigations and public health efforts to combat overdose epidemics.