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Related Concept Videos

¹H NMR: Complex Splitting01:13

¹H NMR: Complex Splitting

A proton M that is coupled to a proton X results in doublet signals for M. However, NMR-active nuclei can be simultaneously coupled to more than one nonequivalent nucleus. When M is coupled to a second proton A, such as in styrene oxide, each peak in the doublet is split into another doublet.
Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied first.
Mass Spectrum01:23

Mass Spectrum

A mass spectrum is the graphical representation of the relative abundance of the charged fragments in an analyte plotted against their mass-to-charge ratio (m/z). The plot's x-axis represents the ratio of the mass of the charged fragment to the number of charges it carries. The y axis of the plot represents the relative abundance of each charged species. The relative abundance is calculated from the signal intensity of each charged species recorded at the detector. The most intense signal (the...

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Related Experiment Video

Updated: Jun 17, 2026

DNA Methylation: Bisulphite Modification and Analysis
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Visualization and Quantification of Single-Base m6A Methylation.

Qiushuang Zhang1,2,3, Yicong Dai1,2, Xucong Teng2,4

  • 1Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing, 100084, China.

Angewandte Chemie (International Ed. in English)
|November 25, 2024
PubMed
Summary
This summary is machine-generated.

Researchers developed a new method called TARS to visualize and quantify N6-methyladenosine (m6A) RNA modifications within single cells at single-base resolution, overcoming previous imaging limitations.

Keywords:
N6-methyladenosineRNA modificationm6A quantificationsingle-base resolutionsingle-cell imaging

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A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
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Area of Science:

  • Molecular Biology
  • Epigenetics
  • RNA Biology

Background:

  • N6-methyladenosine (m6A) is the most abundant RNA modification, playing critical roles in various cellular processes.
  • Accurate detection and quantification of m6A are essential for understanding disease mechanisms and developing therapeutics.
  • Current methods lack the capability for in situ visualization of intracellular m6A at single-base resolution.

Purpose of the Study:

  • To develop a novel method for precise visualization and quantification of m6A RNA modifications within single cells.
  • To achieve single-base resolution imaging of both adenosine (A) and N6-methyladenosine (m6A) at specific RNA sites.

Main Methods:

  • Introduction of the TadA8.20-assisted N6-methyladenosine RNA imaging at single-base resolution (TARS) method.
  • Application of TARS for in situ imaging and quantification of m6A modifications in MALAT1 lncRNA and CCND1 mRNA.
  • Validation in HeLa cells and breast cancer cell lines.

Main Results:

  • TARS enables precise visualization and quantification of m6A modifications at single-base resolution within single cells.
  • Demonstrated high specificity and efficiency in mapping and quantifying m6A on MALAT1 lncRNA and CCND1 mRNA.
  • Successfully visualized both A and m6A forms at specific RNA sites.

Conclusions:

  • TARS is a novel and powerful tool for advancing m6A RNA modification research.
  • Provides accurate and detailed insights into m6A modifications at the single-base level.
  • Facilitates deeper understanding of m6A's role in physiological and pathological conditions.