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Detection of Serum Antibodies Targeting the Marburg Virus Glycoprotein Using a Multiplex Immunoassay Platform
Aquena Ball1, Teri Ann S Wong1, Axel T Lehrer2
1Department of Tropical Medicine, Medical Microbiology, and Pharmacology, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, HI, USA.
Abstract:
Multiplex immunoassays allow for the simultaneous detection of antibody populations targeting multiple antigens in a very sensitive, high-throughput format. This assay has applications in seroprevalence, serodiagnosis, and vaccine assessment with greater sensitivity and dynamic range than standard ELISAs (enzyme-linked immunosorbent assays). Here, we show in detail the methods for multiplex immunoassays to detect IgG to the Marburg virus glycoprotein.
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