Quantification of Filovirus Soluble Glycoprotein Using a Sandwich ELISA

Sandwich enzyme-linked immunosorbent assays (ELISAs), which quantify antigens captured between two layers of antibodies, are sensitive and fundamental tools used to diagnose diseases, evaluate the effects of countermeasures, and quantify target proteins. Many filoviruses use transcriptional editing to express three different glycoproteins (GPs) from the GP gene. The main product transcribed from the GP gene, the soluble glycoprotein (sGP), is detected at high levels in the blood during the acute phase of infection and correlates with viremia, making it an attractive target for molecular diagnostics. Here, we describe a sandwich ELISA-based method for quantifying filovirus sGP.