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Irisin-regulated lncRNAs and their potential regulatory functions in chondrogenic differentiation of human

Yijie Chen1, Wenqi Sha1, Yifan Zhang1

  • 1Department of Otolaryngology-Head and Neck Surgery, The Second Affiliated Hospital of Xi'an Jiaotong University, Shaanxi, 710004, People's Republic of China.

Open Medicine (Warsaw, Poland)
|November 26, 2024
PubMed
Abstract

Insights

Irisin, a myokine, influences chondrogenic differentiation by regulating long noncoding RNAs (lncRNAs) in human mesenchymal stem cells (MSCs). This study identifies key lncRNAs involved in irisin-mediated pathways relevant to osteoarthritis (OA) treatment.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Osteoarthritis (OA) involves dysregulated chondrogenic differentiation.
  • The myokine irisin shows therapeutic potential for OA, but its mechanism is unclear.
  • Long noncoding RNAs (lncRNAs) are critical regulators of chondrocyte differentiation.

Purpose of the Study:

  • To investigate the role of lncRNAs in mediating irisin-induced chondrogenic differentiation.
  • To identify irisin-regulated lncRNAs in human mesenchymal stem cells (MSCs).

Main Methods:

  • Analyzed whole transcriptome sequencing data of irisin-treated human MSCs.
  • Predicted lncRNA targets and competitive endogenous RNA (ceRNA) interactions.
  • Performed functional enrichment analysis of differentially expressed genes (DEGs) and lncRNAs (DElncRNAs).

Main Results:

  • Identified numerous differentially expressed lncRNAs (DElncRNAs) induced by irisin.
  • Highlighted top irisin-induced lncRNAs (e.g., XIST, PAX8-AS1, CASC15, LINC01618, DLX6-AS1) linked to OA and chondrogenesis.
  • Found DEGs co-expressed with DElncRNAs enriched in pathways for skeletal development, extracellular matrix (ECM) organization, cell adhesion, and inflammation.
  • Suggested lncRNAs function as ceRNAs regulating mRNAs like ROR2 and SORBS1.

Conclusions:

  • Demonstrated global regulation of lncRNAs by irisin during human MSC chondrogenic differentiation.
  • Further research is needed to fully characterize the specific roles of key irisin-regulated lncRNAs in chondrogenesis.
Keywords:
ceRNAschondrogenic differentiationco-expressionirisinlncRNAs

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