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Assessing the predictive potential of ADAM8 for disease control in chronic rhinosinusitis with nasal polyps
Peiqiang Liu1,2, Meng Liu1,2, Yibin Sun1,2
1Department of Otolaryngology-Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan, China.
Background:
A disintegrin and metalloproteinase 8 (ADAM8) has been implicated in eosinophilic inflammation; however, its role in chronic rhinosinusitis with nasal polyps (CRSwNP) remains to be elucidated. This study aimed to investigate the predictive significance of ADAM8 levels in nasal secretions for the endotypes and disease control status of CRSwNP.
Methods:
A cohort comprising 120 CRSwNP patients and 45 healthy controls (HCs) was assembled, delineating 53 non-eosinophilic CRSwNP (neCRSwNP) and 67 eosinophilic CRSwNP (eCRSwNP) patients. Immunohistochemistry and enzyme-linked immunosorbent assay (ELISA) were utilized to measure ADAM8 levels in nasal mucosal tissues and secretions from all participants. The receiver operating characteristic (ROC) curves and Pearson correlation analysis were employed to assess the predictive capability of ADAM8 levels in predictiving CRSwNP endotypes and disease control status.
Results:
ADAM8 levels in nasal secretions were elevated in CRSwNP patients compared to HCs, with a more pronounced increase observed in eCRSwNP patients. Elevated ADAM8 concentrations in nasal secretions were positively correlated with peripheral blood eosinophil counts and percentages, tissue eosinophil counts, serum total IgE, Lund-Mackay scores, and Lund-Kennedy scores. Ultimately, 103 CRSwNP patients completed the follow-up protocol, with 72 classified as the controlled group and 31 as the uncontrolled group. Uncontrolled CRSwNP patients exhibited significantly higher ADAM8 levels in nasal secretions compared to the controlled group. The ROC curves indicated that ADAM8 in nasal secretions exhibits robust discriminatory capacity for eCRSwNP and postoperative disease control status.
Conclusion:
ADAM8 in nasal secretions emerges as a potential novel biomarker for the prognostication of CRSwNP endotypes and the postoperative disease control status.
Insights
A disintegrin and metalloproteinase 8 (ADAM8) in nasal secretions can predict chronic rhinosinusitis with nasal polyps (CRSwNP) endotypes and disease control. Higher ADAM8 levels indicate eosinophilic CRSwNP and poorer postoperative outcomes.
Area of Science:
- Immunology
- Otorhinolaryngology
- Biomarker Discovery
Background:
- A disintegrin and metalloproteinase 8 (ADAM8) is linked to inflammation.
- The role of ADAM8 in chronic rhinosinusitis with nasal polyps (CRSwNP) requires further investigation.
Purpose of the Study:
- To assess the predictive value of ADAM8 levels in nasal secretions for CRSwNP endotypes.
- To determine if ADAM8 can predict disease control status in CRSwNP patients.
Main Methods:
- Quantified ADAM8 levels in nasal secretions and tissues using ELISA.
- Compared ADAM8 levels between CRSwNP patients (n=120) and healthy controls (n=45).
- Utilized ROC curves and correlation analysis to evaluate predictive capabilities.
Main Results:
- ADAM8 levels were significantly higher in CRSwNP patients, particularly in the eosinophilic subtype (eCRSwNP).
- Elevated ADAM8 correlated with eosinophil counts, IgE levels, and disease severity scores.
- Higher ADAM8 levels were observed in uncontrolled CRSwNP and predicted poor postoperative outcomes.
Conclusions:
- ADAM8 in nasal secretions is a potential biomarker for CRSwNP endotyping.
- ADAM8 may serve as a novel prognostic marker for disease control and postoperative status in CRSwNP.
