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Updated: May 4, 2026
Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
Published on: May 20, 2013
Rapid Identification of Chemical Constituents in New Compound Aloe Capsule Based on UPLC-Q-Orbitrap-MS
Siyue Chen1, Yajie Dou1, Shumin Zhang1
1School of Chinese Material Medica, Tianjin University of Traditional Chinese Medicine, Tianjin, China.
Rationale:
The chemical composition of the new compound aloe capsule (NCAC) has not been fully elucidated to date, which poses challenges for pharmacology, scientific, and rational drug use. In this study, a rapid and sensitive method was established to comprehensively study the constituents in the NCAC. The contents of the NCAC were analyzed by UPLC-Q-Orbitrap-MS and data postprocessing technology.
Methods:
Firstly, the extract was separated by UPLC system, and the fragment information was obtained under positive and negative ions and then compared with the characteristic fragments described in the literature, so as to achieve the purpose of rapid identification of the compound of NCAC.
Results:
A total of 56 compounds including 21 chromones, 6 pyranones, 7 anthraquinones, 7 anthrone, 5 alkaloids, 4 amino acids, and 6 other components were detected in NCAC by comparing the retention time and mass spectrometry information and retrieving the reference literature.
Conclusions:
The UPLC-Q-Orbitrap-MS method was developed and utilized successfully to identify the major constituents in NCAC and would be helpful for improving the quality control standard of NCAC.
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