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In Vitro Multiparametric Cellular Analysis by Micro Organic Charge-modulated Field-effect Transistor Arrays

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Spatiotemporal Cell Control via High-Precision Electronic Regulation of Microenvironmental pH.

Xiaoyu Zhang¹, Xin Zhang¹, Sizhe Cheng²

¹Department of Mechanical and Industrial Engineering, University of Massachusetts Amherst, Amherst, Massachusetts 01003, United States.

|November 26, 2024

Summary

Scientists created a novel device for precise, real-time control of microenvironmental pH. This breakthrough enables new research in cell biology and tissue engineering by overcoming limitations of traditional pH-modulating methods.

Keywords:

dynamic cell behavior control microelectrolytic pH control microenvironmental pH regulation nanoelectronic pH sensing real-time pH regulation

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In vitro Monitoring of Extracellular pH in Real-Time

In vitro Monitoring of Extracellular pH in Real-Time

Published on: June 3, 2021

Area of Science:

Biotechnology
Cell Biology
Microfluidics

Background:

Accurate extracellular pH regulation is vital for cell functions.
Existing pH control methods lack precision, speed, and spatiotemporal resolution.

Purpose of the Study:

To develop a microfabricated device for precise, localized, and real-time control of microenvironmental pH.
To demonstrate the device's capability in modulating cellular behaviors.

Main Methods:

A microfluidic device employing microelectrolysis for pulsatile pH modulation.
Integration of graphene-electronic pH sensing operating in antiphase with modulation.
Synchronization strategy for coordinated pH control and sensing.

Main Results:

Achieved high-precision pH regulation (<0.1 pH units) with excellent temporal resolution.
Demonstrated real-time control of bacterial motility.
Showcased regulation of cardiomyocyte calcium signaling and necrotic injury.

Conclusions:

The developed device offers a significant advancement over traditional pH control methods.
This technology has broad potential applications in cell biology, physiology, tissue engineering, and regenerative medicine.