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Clinical Applications of Epidermal Stem Cells

Clinical Applications of Epidermal Stem Cells

Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own...

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Related Experiment Video

Updated: Jun 6, 2025

Minced Tissue in Compressed Collagen: A Cell-containing Biotransplant for Single-staged Reconstructive Repair

Minced Tissue in Compressed Collagen: A Cell-containing Biotransplant for Single-staged Reconstructive Repair

Published on: February 24, 2016

Fast Autograft Generation Using Transferable 3D Keratinocyte Cell Sheet on PEDOT:PSS Composite PDMS Membrane for

Yeongseok Jang^1,2, Dongwon Lee³, Jonghyun Oh⁴

¹Department of Mechanical Design Engineering, Jeonbuk National University, Jeonju-si, Jeollabuk-do, 54896, Republic of Korea.

Small (Weinheim an Der Bergstrasse, Germany)

|November 26, 2024

Summary

This study introduces a novel cell sheet technology using mechanical and electrical stimulation for enhanced wound healing. This innovation promotes faster tissue regeneration and improved cell bioactivity in regenerative medicine.

Keywords:

PEDOT:PSS composite autograft keratinocyte transferable cell sheet wound healing

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Last Updated: Jun 6, 2025

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Creation and Transplantation of an Adipose-derived Stem Cell ASC Sheet in a Diabetic Wound-healing Model

Creation and Transplantation of an Adipose-derived Stem Cell ASC Sheet in a Diabetic Wound-healing Model

Published on: August 4, 2017

Area of Science:

Regenerative Medicine
Biomaterials Science
Tissue Engineering

Background:

Cell sheet technology preserves extracellular matrix (ECM) and dense cell tissue for wound healing.
Conventional cell sheets face challenges like middle-layer necrosis due to poor nutrient and oxygen diffusion.
Existing methods require improvements for enhanced cell sheet stability and bioactivity.

Purpose of the Study:

To develop and validate a novel cell sheet technology using mechanical and electrical stimulation.
To enhance cell sheet transfer, bioactivity, and wound healing capabilities.
To overcome limitations of conventional cell sheets in regenerative medicine.

Main Methods:

Development of a micro-structured membrane capable of mechanical and electrical stimulation.
Application of mechanical and electrical stimulation to cell sheets.
In vitro and in vivo validation of the micro-structured membrane's performance.
Comparison with flat membranes under electrical stimulation.

Main Results:

The micro-structured membrane facilitated cell sheet detachment and enhanced cell bioactivity.
Electrical stimulation accelerated angiogenesis and re-epithelialization in wound models.
The novel approach demonstrated superior performance compared to conventional methods.
In vitro and in vivo models confirmed the efficacy of the proposed technology.

Conclusions:

The innovative cell sheet technology significantly enhances rapid wound healing.
Mechanical and electrical stimulation of micro-structured membranes offers a promising approach in regenerative medicine.
This technology addresses nutrient/oxygen diffusion issues and improves cell sheet integration for better therapeutic outcomes.