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Parasexual process in the yeast Yarrowia lipolytica
Journal of Basic Microbiology
|January 1, 1986
Summary
Diploid yeast strains (Yarrowia lipolytica) are unstable, undergoing haploidization. Researchers developed a screening system to show parasexual processes occur alongside the sexual life cycle in these yeast genetics studies.
Area of Science:
- Microbiology
- Genetics
- Yeast Biology
Background:
- Diploid strains of Yarrowia lipolytica exhibit instability during their life cycle.
- This instability leads to the production of mitotic segregants through haploidization.
Purpose of the Study:
- To investigate the mechanisms of genetic instability in Yarrowia lipolytica.
- To develop a screening system for identifying parasexual processes in yeast.
- To analyze the role of aneuploid intermediates in the haploidization process.
Main Methods:
- Development of a specialized screening system.
- Statistical analysis of segregation patterns.
- Segregation data analysis of yeast clones.
- Selection of mitotic segregants based on 2-deoxyglucose resistance.
Main Results:
- Parasexual processes were confirmed to occur in Yarrowia lipolytica, in addition to its sexual life cycle.
- Haploidization proceeds through aneuploid intermediates, statistically supported by segregation data.
- The direction of genetic crosses did not influence the selection of mitotic segregants.
Conclusions:
- Yarrowia lipolytica diploid strains are genetically unstable and undergo haploidization via parasexual processes.
- Aneuploid intermediates play a crucial role in the observed haploidization mechanism.
- The developed screening system is effective for studying parasexual reproduction in yeast.