Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

87.5K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
87.5K
CRISPR01:59

CRISPR

49.5K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
49.5K
What is Genetic Engineering?00:49

What is Genetic Engineering?

73.6K
Overview
73.6K
Overview of DNA Repair02:25

Overview of DNA Repair

30.4K
In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
30.4K
Base Excision Repair01:54

Base Excision Repair

22.0K
One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template.
The first step of...
22.0K
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

1.8K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
1.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>In vitro</i> and <i>in vivo</i> evaluation of DNA-integrated diclofenac-HPMC hydrogel for enhanced ocular anti-inflammatory drug delivery.

Journal of biomaterials science. Polymer edition·2026
Same author

The skin microbiome-immune-barrier axis: implications for inflammatory skin disorders and immunotherapeutic strategies.

Immunotherapy·2026
Same author

Development of HPMC-modified starch/ZnO nanocomposite films using biodegradable plasticizers for improved antioxidant, antimicrobial, and cytocompatible characteristics.

Journal of biomaterials science. Polymer edition·2026
Same author

DNA-driven enhancement of ocular drug delivery: formulation and evaluation of diclofenac-loaded HPMC films.

Journal of biomaterials science. Polymer edition·2025
Same author

Development of ZnO-resistant starch prebiotic matrix conjugate for metronidazole delivery for enhanced probiotic viability and antimicrobial activity.

International journal of biological macromolecules·2025
Same author

Insightful Perspectives on Sodium-glucose Co-transporter 2 Inhibitors: Navigating Safety Updates and Beyond.

Current drug research reviews·2025

Related Experiment Video

Updated: Jun 6, 2025

DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications
08:59

DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications

Published on: September 27, 2019

11.5K

Revolutionizing DNA: advanced modification techniques for next-gen nanotechnology.

Pratikeswar Panda1, Rajaram Mohapatra1

  • 1Department of Pharmaceutics, School of Pharmaceutical Science, Siksha 'O' Anusandhan University, Bhubaneswar, Odisha, India.

Nucleosides, Nucleotides & Nucleic Acids
|November 26, 2024
PubMed
Summary
This summary is machine-generated.

Advanced DNA modification and coupling techniques are enabling sophisticated DNA nanotechnology for innovations in healthcare and materials science. These methods allow for precise engineering of DNA nanostructures with tailored functionalities.

Keywords:
DNADNA nanostructuresbiotinylationcoupling strategiesmodification techniques

More Related Videos

Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures
08:15

Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures

Published on: June 26, 2020

4.2K
A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
10:07

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells

Published on: August 25, 2017

7.7K

Related Experiment Videos

Last Updated: Jun 6, 2025

DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications
08:59

DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications

Published on: September 27, 2019

11.5K
Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures
08:15

Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures

Published on: June 26, 2020

4.2K
A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
10:07

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells

Published on: August 25, 2017

7.7K

Area of Science:

  • Biotechnology
  • Nanotechnology
  • Materials Science

Background:

  • DNA modification and coupling technologies are rapidly advancing.
  • These advancements are crucial for the development of DNA nanotechnology.

Purpose of the Study:

  • To explore diverse DNA modification and coupling strategies.
  • To highlight their role in creating advanced DNA nanostructures and enabling innovations.

Main Methods:

  • Utilizing various chemical handles (e.g., amino, thiol, alkyne, azide) and coupling reactions (e.g., Palladium-Catalyzed Couplings).
  • Implementing modifications such as hydrophobic alterations, redox-active moieties, crosslinking, and Biotinylation.
  • Leveraging next-generation sequencing (NGS)-based DNA modifications.

Main Results:

  • Diverse coupling strategies enable the construction of intricate DNA nanostructures.
  • Modifications significantly enhance DNA's functional repertoire, allowing precise control over structure and interactions.
  • DNA nanotechnology demonstrates unprecedented precision and functionality.

Conclusions:

  • Advanced DNA modification and coupling techniques are key drivers of DNA nanotechnology.
  • These techniques unlock transformative applications in drug delivery, diagnostics, and bioengineering.
  • DNA's versatility as a programmable biomaterial is showcased, ushering in a new era of technological advancement.