GC-MS- and LC-TOF-MS/MS-based ginger volatile oil serum analysis and the potential mechanism of the anticancer effect of serum component citral on MCF-7 breast cancer cells
Contact us if these videos are not relevant.
Contact us if these videos are not relevant.
View abstract on PubMed
Summary
This summary is machine-generated.Ginger volatile oil (GVO) components in rat blood were analyzed. Citral in GVO demonstrated significant anti-breast cancer effects by inhibiting cell proliferation and inducing apoptosis, clarifying GVO
Area Of Science
- Pharmacology
- Metabolomics
- Cancer Research
Background
- Ginger volatile oil (GVO) has potential therapeutic properties.
- Understanding GVO's metabolites and mechanisms is crucial for its application.
- Breast cancer remains a significant global health concern.
Purpose Of The Study
- To identify blood components and metabolites of GVO in rats.
- To investigate the anti-breast cancer effects of GVO components.
- To elucidate the molecular mechanisms of GVO against breast cancer.
Main Methods
- Gas chromatography-time-of-flight mass spectrometry and UPLC-Q-TOF-MS were used to analyze rat serum.
- Network pharmacology was employed to predict active components and pathways.
- In vitro experiments using MCF-7 breast cancer cells assessed proliferation and apoptosis.
Main Results
- 34 differential metabolites were identified, with citral being a key component.
- GVO-medicated serum and citral inhibited breast cancer cell proliferation and migration.
- Citral significantly increased early and late apoptosis in breast cancer cells.
Conclusions
- This study clarifies the material basis of GVO's anti-breast cancer activity.
- The findings highlight citral as a primary active component of GVO.
- The combined approach provides insights into GVO's molecular mechanisms against breast cancer.
Contact us if these videos are not relevant.
Contact us if these videos are not relevant.