DMS-MapSeq Analysis of Antisense Oligonucleotide Binding to lncRNA PANDA

While various methods exist for examining and visualizing the structures of RNA molecules, dimethyl sulfate-mutational profiling and sequencing (DMS-MaPseq) stands out for its simplicity and versatility. This technique has been proven effective for studying RNA structures both in vitro and in complex biological settings. We present an updated protocol of DMS-MaPseq, as well as methodology that enables it to be used for detection of antisense oligonucleotides (ASOs) binding to RNA. By applying this protocol, we successfully characterized the structural ensemble of the HIV1 Rev Response Element (RRE), along with its two alternative structures. The findings align with previously published research validating the accuracy of the method. We also demonstrate the utility of the DMS-MaPseq protocol by resolving and confirming ASO binding at the complementary sites of the P21-associated noncoding RNA DNA damage-activated (PANDA) long non-coding RNA via decreased DMS reactivity. © 2024 Wiley Periodicals LLC. Basic Protocol 1: DMS-MaPseq on HIV1-RRE Basic Protocol 2: DMS-MaPseq on PANDA with ASO probing.