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Updated: Jun 6, 2025

Using Expansion Microscopy to Physically Enlarge Whole-Mount Drosophila Embryos for Super-Resolution Imaging
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High-throughput expansion microscopy enables scalable super-resolution imaging.

John H Day1, Catherine M Della Santina1, Pema Maretich2

  • 1Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, United States.

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|November 26, 2024
PubMed
Summary
This summary is machine-generated.

High-throughput Expansion Microscopy (HiExM) allows nanoscale imaging of many samples at once in 96-well plates. This scalable super-resolution technique improves detection of cellular changes, like drug effects on heart cells.

Keywords:
A549 cellscardiomyocytescell biologycell culturehuman

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Area of Science:

  • Cell Biology
  • Microscopy Techniques
  • Biotechnology

Background:

  • Expansion microscopy (ExM) offers nanoscale imaging via specimen expansion.
  • Current ExM methods have limited throughput for simultaneous sample processing.

Purpose of the Study:

  • To develop a high-throughput platform for expansion microscopy (HiExM).
  • To enable scalable super-resolution imaging in standard 96-well plates.

Main Methods:

  • Cells were cultured and processed in 96-well plates for HiExM.
  • HiExM achieved approximately 4.2x isotropic expansion.
  • Combined HiExM with high-throughput confocal imaging.

Main Results:

  • Demonstrated successful expansion across multiple wells and plates.
  • HiExM detected dose-dependent effects of doxorubicin on cardiomyocyte nuclear DNA, unseen in unexpanded samples.
  • Showcased improved detection of cellular phenotypes in response to drug treatment.

Conclusions:

  • HiExM significantly enhances the scalability and ease of ExM.
  • This method improves the detection of subtle cellular changes for drug screening and biological research.
  • HiExM broadens the application of ExM for scalable super-resolution imaging.