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Updated: Jun 6, 2025
10:41
Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations
Published on: March 29, 2017
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Single Nucleotide Recognition and Mutation Site Sequencing Based on a Barcode Assay and Rolling Circle Amplification
Linmin Zhong1, Huiping Chen1, Shuang Cao2
1Department of Health Inspection and Quarantine, School of Public Health, Fujian Medical University, Fuzhou 350122, China.
Biosensors
|November 26, 2024
Summary
This study introduces a new barcode method for precise single nucleotide polymorphism (SNP) detection. This highly sensitive approach enables accurate microbial sequencing and mutation analysis.
Area of Science:
- Molecular Biology
- Genomics
- Biotechnology
Background:
- Single nucleotide polymorphisms (SNPs) pose challenges for microbial detection and treatment.
- Advanced sequencing technologies are needed to overcome these challenges.
Purpose of the Study:
- To develop a novel, highly sensitive method for single nucleotide recognition.
- To enable precise identification and analysis of base changes at individual loci.
Main Methods:
- A barcode-based approach using dual-head folded complementary template probes and DNA ligase for base identification.
- Rolling circle amplification (RCA) coupled with CdSe quantum dot self-assembly for single-particle fluorescence readout.
- Bio-barcode array analysis for screening multiple base change sites.
Main Results:
- The method demonstrated high precision, sensitivity, and single nucleotide resolution.
- Accurate sequencing of a drug-resistant mutation site in *Helicobacter pylori* (*H. pylori*) was achieved.
- The approach showed excellent stability in mutation detection.
Conclusions:
- This novel barcode-based method offers a promising next-generation sequencing technology.
- It addresses the challenges posed by SNPs in microbial detection and treatment.
- The technique provides a robust platform for precise genetic analysis.
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