Exploring Plasmonic Standalone Surface-Enhanced Raman Scattering Nanoprobes for Multifaceted Applications in...

Surface-enhanced Raman scattering (SERS) is an innovative spectroscopic technique that amplifies the Raman signals of molecules adsorbed on rough metal surfaces, making it pivotal for single-molecule detection in complex biological and environmental matrices. This review aims to elucidate the design strategies and recent advancements in the application of standalone SERS nanoprobes, with a special focus on quantifiable SERS tags. We conducted a comprehensive analysis of the recent literature, focusing on the development of SERS nanoprobes that employ novel nanostructuring techniques to enhance signal reliability and quantification. Standalone SERS nanoprobes exhibit significant enhancements in sensitivity and specificity due to optimized hot spot generation and improved reporter molecule interactions. Recent innovations include the development of nanogap and core-satellite structures that enhance electromagnetic fields, which are crucial for SERS applications. Standalone SERS nanoprobes, particularly those utilizing indirect detection mechanisms, represent a significant advancement in the field. They hold potential for wide-ranging applications, from disease diagnostics to environmental monitoring, owing to their enhanced sensitivity and ability to operate under complex sample conditions.