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Updated: May 8, 2026

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries
Published on: February 1, 2011
Development of a non-targeted metabolomics-based screening method for elucidating the metabolic characteristics and
Dandan Wang1, Jianxia Yao1, Lu Li1
1Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, China; Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Hohhot, Inner Mongolia 010018, China; Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, China.
Abstract:
The increasing demand for healthy foods has led to widespread interest in lactic acid bacteria due to their potential health benefits. We propose the hypothesis that Lacticaseibacillus paracasei (L. paracasei) can produce beneficial metabolites under specific conditions, which offers potential applications in functional foods. In this study, we analyzed the fermentation supernatants and brown fermented milk metabolites of L. paracasei to identify those with possible applications in functional foods, which have great potential. We found that L. paracasei IMAU32642 produced unique metabolites, including docosahexaenoic acid (DHA), leucyl phenylalanine, and oleic acid in its fermentation supernatant. Meanwhile, L. paracasei IMAU60048 exhibited unique application prospects in brown fermented milk, with higher yields of arachidonic acid and caprylic acid compared to other strains. This study offers a new and effective method for screening L. paracasei. The study can promote the development of functional foods and enhance their health value.
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