Transcriptomic data of peach varieties with different chilling requirement levels

Area of Science:

• Plant Science
• Genomics
• Molecular Biology

Background:

• Peach (Prunus persica) cultivation requires bud endodormancy release, triggered by cold accumulation known as chilling requirement (CR).
• CR is a critical factor influencing flowering and fruiting, exhibiting significant genotypic variation.
• Understanding the genetic basis of CR is essential for improving peach breeding and cultivation.

Purpose of the Study:

• To investigate the transcriptomic differences in peach buds with varying chilling requirement (CR) levels.
• To identify potential genes associated with CR and bud endodormancy in peaches.
• To provide a valuable dataset for future functional genomics studies on peach CR.

Main Methods:

• RNA sequencing was performed on peach buds from two genotypes with different CR levels across three endodormancy stages.
• 18 RNA-seq libraries were generated, and sequencing data were mapped to the reference genome after quality control.
• Differential gene expression analysis identified 2,481 unique differentially expressed genes (DEGs) and functional annotation was conducted.

Main Results:

• Transcriptomic analysis revealed significant gene expression variations between peach buds with different CR levels.
• A substantial number of differentially expressed genes (DEGs) were identified, suggesting their potential roles in CR.
• Functional annotation provided insights into the biological pathways and functions associated with CR.

Conclusions:

• Peach bud gene expression profiles differ based on chilling requirement (CR) levels.
• The identified DEGs represent potential candidates for genes regulating CR and bud endodormancy.
• This transcriptomic dataset serves as a foundation for further research into the genetic mechanisms of CR in peaches.