Molecular characterization of human respiratory syncytial virus strains circulating among hospitalized children in

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An In vitro Model to Study Immune Responses of Human Peripheral Blood Mononuclear Cells to Human Respiratory Syncytial Virus Infection
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Ashraf I Khasawneh1, Nisreen Himsawi2, Ashraf Sammour3

  • 1Department of Microbiology, Pathology, and Forensic Medicine, Faculty of Medicine, The Hashemite University, Zarqa, 13133, Jordan. ashrafkh@hu.edu.jo.

BMC Infectious Diseases
|November 26, 2024
PubMed

Insights

This study identified Human Respiratory Syncytial Virus (RSV) strains in hospitalized children in Jordan, finding RSV-A ON1 and RSV-B BA9 genotypes predominate. Understanding these RSV genotypes is crucial for developing effective vaccines and treatments.

Area of Science:

  • Virology
  • Epidemiology
  • Public Health

Background:

  • Human Respiratory Syncytial Virus (HRSV) causes severe pediatric respiratory infections, leading to hospitalizations.
  • The virus exhibits significant antigenic diversity due to high mutagenic potential, complicating vaccine development.
  • This study investigated the prevalence and molecular characteristics of RSV in Jordan.

Purpose of the Study:

  • To determine the epidemiological prevalence of RSV in hospitalized children in Jordan.
  • To characterize the molecular and phylogenetic features of circulating RSV strains.
  • To provide data for improved RSV disease management and vaccine strategies.

Main Methods:

  • Collected 288 nasopharyngeal swabs from hospitalized children in Amman, Jordan (2022-2023).
  • Screened samples for respiratory pathogens using PCR.
  • Amplified the RSV G gene for molecular characterization and phylogenetic analysis.

Main Results:

  • RSV was detected in 12.2% (35/288) of samples.
  • RSV-A (71.4%) and RSV-B (11.4%) were identified, with RSV-A ON1 and RSV-B BA9 genotypes predominating.
  • Observed multiple substitutions in the G gene, potentially affecting antigenicity and pathogenicity.

Conclusions:

  • Updated epidemiological data on circulating RSV strains in Amman, Jordan.
  • Molecular characterization reveals predominant genotypes and potential for antigenic variation.
  • Continuous RSV surveillance is vital for vaccine development and public health interventions.
Abstract

Keywords:
BA9JordanPhylogeneticRSVRT-PCR, ON1Respiratory

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