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Metabolites and Metabolic Functional Changes-Potential Markers for Endothelial Cell Senescence.
Jingyuan Ya1, Alison Whitby2, Ulvi Bayraktutan1,3
1Stroke, Academic Unit of Mental Health and Clinical Neurosciences, School of Medicine, Queens Medical Centre, University of Nottingham, Nottingham NG7 2UH, UK.
Biomolecules
|November 27, 2024
Summary
Senescent endothelial cells (ECs) contribute to vascular aging. Metabolite profiling identified L-proline, L-glutamate, NAD+, and taurine as potential biomarkers for detecting these aging cells.
Area of Science:
- Biochemistry
- Cell Biology
- Gerontology
Background:
- Senescent endothelial cells (ECs) accumulate in vasculature, driving vascular aging and related diseases.
- Identifying senescent cells via novel biomarkers is crucial for preventing age-related diseases and improving health.
- Metabolites are key biological pathway components and reliable indicators of cellular processes.
Purpose of the Study:
- To identify novel metabolic biomarkers for senescent endothelial cells.
- To investigate metabolic differences between young and senescent human brain microvascular endothelial cells (HBMECs).
Main Methods:
- Senescent HBMECs were generated and characterized by morphological, nuclear, and enzymatic changes.
- Comparative metabolic profiling of intracellular and secreted metabolites from young versus senescent HBMECs was performed.
- Endothelial characteristics, including CD31/PECAM-1 expression, were assessed.
Main Results:
- Senescent HBMECs exhibited loss of endothelial characteristics, with CD31/PECAM-1 disappearing from cell junctions.
- Significant differences in glucose, glutamine, and fatty acid metabolism were observed between young and senescent HBMECs.
- L-proline, L-glutamate, NAD+, and taurine/hypotaurine pathway components were identified as potential biomarkers.
Conclusions:
- Metabolic profiling can reveal key differences in aging endothelial cells.
- Specific metabolites like L-proline, L-glutamate, NAD+, and taurine show promise as biomarkers for senescent ECs.
- Further research is needed to validate these metabolites as biomarkers and potential therapeutic targets for vascular aging.
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