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Liver Fluke-Derived Molecules Accelerate Skin Repair Processes in a Mouse Model of Type 2 Diabetes Mellitus
Anna Kovner1, Yaroslav Kapushchak1, Oxana Zaparina1
1Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (ICG SB RAS), 10 Akad. Lavrentiev Ave., Novosibirsk 630090, Russia.
International Journal of Molecular Sciences
|November 27, 2024
Summary
Bioactive molecules from the liver fluke Opisthorchis felineus, including excretory-secretory products (ESP) and eggs, significantly accelerate chronic wound healing in diabetic mice. These compounds reduce inflammation and promote tissue repair, offering a novel therapeutic approach.
Area of Science:
- Biochemistry
- Parasitology
- Dermatology
- Regenerative Medicine
Background:
- Chronic nonhealing wounds, like diabetic ulcers, represent a significant clinical challenge.
- Existing treatments are limited, necessitating novel therapeutic strategies.
- Bioactive molecules from Opisthorchis felineus have shown potential in acute wound healing.
Purpose of the Study:
- To investigate the wound-healing properties of Opisthorchis felineus excretory-secretory products (ESP) and inactivated eggs.
- To evaluate these properties in a type 2 diabetes mellitus mouse model.
- To assess the effects on inflammation, angiogenesis, and extracellular matrix remodeling.
Main Methods:
- Superficial wounds were inflicted on db/db mice (type 2 diabetes model).
- Mice were treated with O. felineus ESP, inactivated eggs, or controls (vehicle, PDGF).
- Histopathology, immunohistochemistry, RT-PCR, and cell proliferation assays were performed.
Main Results:
- ESP and egg treatments significantly increased wound healing percentage compared to controls after 14 days.
- Treatments reduced inflammation (M1-to-M2 polarization shift), modulated vascular response, and promoted extracellular matrix remodeling.
- ESP stimulated keratinocyte proliferation in vitro, while eggs did not.
Conclusions:
- Bioactive molecules from O. felineus demonstrate significant wound-healing potential in a diabetic model.
- These molecules modulate key processes involved in tissue repair, comparable to PDGF.
- O. felineus derivatives offer promising avenues for developing new therapies for chronic nonhealing wounds.
