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Related Experiment Video

Updated: Jun 6, 2025

Author Spotlight: Accelerating Diagnostic Accuracy with Direct Identification of Gram-Negatives from Blood Culture Bottles

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Published on: May 24, 2024

Improving Turnaround Times for Routine Antimicrobial Sensitivity Testing Following European Committee on

Raewyn Edmondson¹, Kordo Saeed^1,2, Steve Green¹

¹Department of Infection, Microbiology, University Hospital Southampton, Tremona Road, Southampton SO16 6YD, UK.

Antibiotics (Basel, Switzerland)

|November 27, 2024

Summary

Rapid antibiotic susceptibility testing (AST) is crucial for treating bacteraemia. This study demonstrates a modified disc diffusion method, reducing incubation time from 24 hours to 6 hours, yielding accurate results for faster patient care.

Keywords:

EUCAST antibiotic susceptibility testing antimicrobial stewardship bacteraemia empiric treatment

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Preparation of a Blood Culture Pellet for Rapid Bacterial Identification and Antibiotic Susceptibility Testing

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Last Updated: Jun 6, 2025

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Published on: July 9, 2012

Preparation of a Blood Culture Pellet for Rapid Bacterial Identification and Antibiotic Susceptibility Testing

Preparation of a Blood Culture Pellet for Rapid Bacterial Identification and Antibiotic Susceptibility Testing

Published on: October 15, 2014

Area of Science:

Clinical Microbiology
Infectious Diseases
Antimicrobial Resistance

Background:

Bacteraemia requires prompt antibiotic intervention; delayed treatment increases mortality and resistance.
Traditional antibiotic susceptibility testing (AST) methods, like disc diffusion, have long turnaround times (over 2 days).
Rapid AST is essential to optimize empiric antibiotic therapy and improve patient outcomes.

Purpose of the Study:

To evaluate a modified disc diffusion method for faster AST results.
To reduce the incubation time for disc diffusion from 16-24 hours to 6 hours.
To assess the accuracy and reliability of 6-hour incubation AST compared to the standard 24-hour method.

Main Methods:

Collected 82 positive blood culture samples between January and April 2022.
Identified organisms using MALDI-ToF and performed disc diffusion AST.
Compared disc diffusion results (zone sizes and susceptibility interpretations) from 6-hour and 24-hour incubation periods.

Main Results:

Achieved 99.65% agreement in interpreted susceptibility readings between 6-hour and 24-hour incubation.
Observed an average zone size difference of only 1.08 mm.
Demonstrated high concordance between the shortened and standard incubation times.

Conclusions:

A 6-hour incubation period for disc diffusion AST provides reliable and accurate results.
This non-automated method significantly reduces turnaround time for AST.
Faster AST enables same-day testing, improving patient management and antimicrobial stewardship.