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Molecular Characterization of miRNAs in Myzus persicae Carrying Brassica Yellows Virus

Meng-Jun He1, Yun Wang1, Mei Zhao1

  • 1Ministry of Agriculture and Rural Affairs Key Laboratory of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing 100193, China.

Biology
|November 27, 2024
PubMed

Insights

Brassica yellows virus (BrYV) significantly alters microRNA (miRNA) expression in the green peach aphid, Myzus persicae. These changes in miRNA profiles highlight potential molecular mechanisms underlying virus transmission by aphids.

Area of Science:

  • Molecular Biology
  • Virology
  • Entomology

Background:

  • MicroRNAs (miRNAs) are key regulators of gene expression at the post-transcriptional level.
  • The role of miRNAs in the interaction between Myzus persicae and Brassica yellows virus (BrYV) is not well understood.
  • Investigating miRNA profiles in aphids under different host plant and virus conditions is crucial for understanding plant-pathogen interactions.

Purpose of the Study:

  • To characterize microRNA (miRNA) profiles in Myzus persicae in response to Brassica yellows virus (BrYV) infection.
  • To identify differentially expressed miRNAs in aphids under varying host plant (turnip vs. Arabidopsis thaliana) and virus (BrYV-free vs. BrYV-carrying) conditions.
  • To elucidate the potential molecular pathways targeted by differentially expressed miRNAs in BrYV-infected aphids.

Main Methods:

  • miRNA profiling was performed on Myzus persicae populations under two distinct treatments: raised on turnip plants and raised on Arabidopsis thaliana.
  • Aphids were analyzed in both non-viruliferous and viruliferous states for each treatment.
  • Differential expression analysis and KEGG enrichment analysis were conducted to identify significant miRNA changes and their targeted pathways.

Main Results:

  • A substantial number of known and novel mature miRNAs were identified across all experimental groups.
  • Significant differential expression of miRNAs was observed in BrYV-carrying aphids, particularly under the Arabidopsis thaliana treatment, with eight upregulated and four downregulated miRNAs.
  • KEGG enrichment analysis indicated that target genes of differentially expressed miRNAs are mainly involved in pathways such as Peroxisome, neuroactive ligand-receptor interaction, and metabolism of xenobiotics by cytochrome P450.

Conclusions:

  • The level of BrYV infection influences miRNA expression patterns in Myzus persicae.
  • Differentially expressed miRNAs in response to BrYV infection target key biological pathways, suggesting their involvement in aphid-virus interactions.
  • These findings provide valuable insights into the molecular mechanisms of BrYV transmission by aphids and open avenues for future research.
Keywords:
bioinformaticsbrassica yellows virusgreen peach aphidmicroRNAspolerovirussRNA sequencing

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