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An Automated Digital Microfluidic System Based on Inkjet Printing.

Wansheng Hu1, Ming Cao2, Lingni Liao2

  • 1School of Microelectronics, Shanghai University, Shanghai 201800, China.

Micromachines
|November 27, 2024
PubMed
Summary

This study introduces an automated inkjet printing system for creating uniform 3D cell spheroids and organoids. This high-throughput method enhances cancer research, drug testing, and personalized medicine applications.

Keywords:
controllable sizeinkjet printingspheroidsuniform preparation

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Area of Science:

  • Biotechnology
  • Cell Biology
  • 3D Cell Culture

Background:

  • Three-dimensional (3D) cell spheroids enhance cellular interactions, viability, and proliferation, crucial for cancer research and drug testing.
  • Spheroid size critically influences cellular functions and drug response, necessitating precise size control for experiments.
  • Conventional spheroid culture methods face challenges in size consistency, uniformity, and throughput.

Purpose of the Study:

  • To develop an automated, intelligent inkjet printing system for precise spheroid size control and high-throughput spheroid generation.
  • To overcome limitations of conventional 3D cell culture techniques.
  • To enable customized spheroid sizes for various drug sensitivity experiments and organoid culture.

Main Methods:

  • An automated inkjet printing system was developed with algorithms to precisely control droplet volume for variable spheroid sizes.
  • A bidirectional compensation method was used to achieve spheroid size uniformity (coefficient of variation <10%).
  • An automatic pipetting module enabled high-throughput spheroid preparation, demonstrated by printing 216 spheroids in 11 minutes.

Main Results:

  • The system successfully generated spheroids with precise size control and high uniformity.
  • High-throughput printing of a 3x3 spheroid array in a 24-well plate was achieved rapidly.
  • Mouse small intestinal organoids were successfully printed and cultured for 7 days, showing potential for further applications.

Conclusions:

  • The developed automated inkjet printing system offers precise control over spheroid size and high-throughput capabilities.
  • This technology significantly advances 3D cell culture for applications in drug screening and personalized medicine.
  • The system's ability to culture organoids highlights its broad potential in biomedical research.