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Cd-resistant Plant Growth-promoting Rhizobacteria Bacillus Siamensis R27 Absorbed Cd And Reduced Cd Accumulation In Lettuce (lactuca Sativa L.).

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Cd-resistant Plant Growth-promoting Rhizobacteria Bacillus Siamensis R27 Absorbed Cd And Reduced Cd Accumulation In Lettuce (lactuca Sativa L.).

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Cd-Resistant Plant Growth-Promoting Rhizobacteria Bacillus siamensis R27 Absorbed Cd and Reduced Cd Accumulation in

Shaofang Liu^1,2,3, Yushan Huang^1,3, Qinyuan Zheng^1,3

¹Key Laboratory of Natural Microbial Medicine Research of Jiangxi Province, Jiangxi Science and Technology Normal University, Nanchang 330013, China.

|November 27, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

Plant growth-promoting rhizobacteria (PGPR) can remediate cadmium (Cd) pollution and boost crop growth. A novel Bacillus siamensis strain (R27) effectively removes Cd and enhances lettuce growth by reducing Cd uptake and increasing antioxidant activity.

Keywords:

Bacillus siamensis Cd-resistant bacteria PGPR bioremediation lettuce

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Area of Science:

Environmental Microbiology
Bioremediation
Plant-Bacterial Interactions

Background:

Cadmium (Cd) contamination in soil poses risks to plant growth and food safety.
Plant growth-promoting rhizobacteria (PGPR) offer a sustainable solution for heavy metal bioremediation and crop enhancement.
Understanding PGPR mechanisms for Cd adsorption and plant stress mitigation is crucial.

Purpose of the Study:

To isolate Cd-resistant PGPR with plant growth-promoting traits from metal-contaminated soil.
To characterize the Cd adsorption mechanisms of an isolated bacterial strain.
To evaluate the strain's efficacy in promoting lettuce growth and mitigating Cd toxicity in contaminated soil.

Main Methods:

Isolation and screening of Cd-resistant bacteria from vegetable rhizosphere.

Characterization of plant growth-promoting traits (phosphate solubilization, IAA, siderophore production).

Identification of the best strain using physiological, biochemical, and molecular methods.

Assessment of Cd removal efficiency, adsorption mechanisms (FTIR, SEM, TEM, EDX), and impact on lettuce growth and Cd uptake (gene expression, BCF, TF).

Main Results:

Isolated six Cd-resistant strains; R27 (identified as Bacillus siamensis) showed highest Cd tolerance (2000 mg/L) and significant PGP traits.
Strain R27 demonstrated 80.1% Cd removal via cell surface adsorption and intracellular accumulation.
Pot experiments showed R27 significantly enhanced lettuce growth, reduced Cd uptake (down-regulation of IRT1, Nramp1, HMA2, HMA4, ZIP4, ZIP12), and increased antioxidant activity.

Conclusions:

Bacillus siamensis R27 is a promising candidate for bioremediation of Cd-polluted soils.
This strain can act as a bio-fertilizer, enhancing plant growth and tolerance to Cd stress.
The study elucidates R27's mechanisms for Cd adsorption and plant protection, offering a dual benefit for contaminated environments.