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Updated: Jun 6, 2025

Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines
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Application of an Efficient Enhancer in Gene Function Research.

Feng-Xian Guo1,2,3, Rui-Xue Yang1,2,3, Xia Yang1,2

  • 1State Key Laboratory of Plant Diversity and Specialty Crops and Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.

Plants (Basel, Switzerland)
|November 27, 2024
PubMed
Summary
This summary is machine-generated.

The active ribosomal RNA complementary (ARC) enhancer significantly boosts gene expression in plants. Six tandem repeats of ARC (6 × ARC) increased expression up to 130-fold, enhancing gene function manifestation and crop engineering potential.

Keywords:
ARCArabidopsis thalianaChirita pumilaenhancer

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Area of Science:

  • Plant Biotechnology
  • Molecular Biology
  • Crop Engineering

Background:

  • Increasing exogenous gene expression is critical for successful genetic transformation and crop engineering.
  • Translational enhancers play a vital role in boosting gene expression levels and achieving desired phenotypes.

Purpose of the Study:

  • To compare the efficiency of three translational enhancers (Ω, OsADH 5', and ARC) in enhancing gene expression in plants.
  • To evaluate the potential of the ARC enhancer, particularly 6 × ARC, for gene function research and crop improvement.

Main Methods:

  • Comparative study of translational enhancers using transient gene expression systems in *Nicotiana benthamiana* and *Chirita pumila*.
  • Evaluation of 6 × ARC efficiency in *agrobacterium*-mediated transformation systems in *C. pumila* and *Arabidopsis thaliana*.
  • Assessing the phenotypic effects of 6 × ARC in conjunction with specific genes (*CpCYC1*, *CpCYC2*, *AtAP1*).

Main Results:

  • Six tandem repeats of ARC (6 × ARC) demonstrated superior enhancing efficiency, increasing expression up to 130 times compared to controls.
  • 6 × ARC significantly amplified phenotypic effects, including repression of stamen development and pigmentation in *C. pumila*.
  • In *Arabidopsis thaliana*, 6 × ARC promoted anthocyanin pigment accumulation in both vegetative and reproductive organs.

Conclusions:

  • The 6 × ARC enhancer shows significant potential for amplifying gene function manifestation in transgenic plants.
  • This enhancer can effectively reveal the complete function of exogenous genes, aiding in gene function research.
  • The 6 × ARC enhancer holds promise for applications in genetic transformation and crop engineering.