A surface-labeled 18 kilodalton antigen in Schistosoma mansoni

A mild surface-labeling procedure was applied to various developmental stages of Schistosoma mansoni. An 18 kDa protein was preferentially labeled in freshly transformed schistosomula. The labeled protein was equally present on skin-penetrated and mechanically prepared schistosomula and it disappeared upon digestion of intact parasites with proteolytic enzymes. The 18 kDa protein could be specifically precipitated with an antiserum raised against 3-h schistosomula. Six-day lung forms also presented a single major labeled protein component, but the apparent molecular weight of this protein in acrylamide gels was higher than 18 000. Fourteen-day-old and adult schistosomes showed only weak labeling distributed in several bands. The radioactivity pattern of adult worms (but not of schistosomula) could also be obtained by incubating fresh parasites in a medium which had previously been used to label schistosomes and to which a 100 000-fold excess of 127I over 125I had been added. Post-labeling incubation of parasites was found to be essential for the detection of stable surface proteins.