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Surface components of Onchocerca volvulus.

D W Taylor, J M Goddard, J E McMahon

    Molecular and Biochemical Parasitology
    |March 1, 1986
    PubMed
    Summary
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    This study developed a rapid method to isolate Onchocerca volvulus microfilariae and identified stage-specific surface proteins on microfilariae, adult worms, and larvae. Human infection serum recognized some adult worm surface proteins, but not microfilarial ones.

    Area of Science:

    • Parasitology
    • Molecular Biology
    • Immunology

    Background:

    • Onchocerca volvulus is a parasitic nematode causing onchocerciasis.
    • Understanding the parasite's surface antigens is crucial for developing diagnostics and vaccines.
    • Previous research has limited characterization of stage-specific surface proteins.

    Purpose of the Study:

    • To develop a rapid isolation technique for Onchocerca volvulus microfilariae.
    • To identify and characterize surface proteins of microfilariae, adult worms, and L3 larvae.
    • To investigate the immunogenicity of these surface proteins using human infection serum.

    Main Methods:

    • Sephadex G25 gel filtration for microfilariae isolation.
    • Surface radiolabeling (Iodogen technique) of different parasite stages.

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  • Protein molecular weight characterization using SDS-PAGE.
  • Western blot analysis and indirect immunofluorescence assays with human infection serum.
  • Fluorescent lectin binding studies.

    • Main Results:

    • A rapid Sephadex G25 gel filtration method was established for isolating live microfilariae.
    • Two uterine microfilarial surface proteins (14.8 kDa, 15 kDa) and one skin microfilarial surface protein (15 kDa) were identified.
    • Multiple surface proteins were characterized on adult worms (10 proteins) and L3 larvae (7 proteins).
    • Human infection serum recognized three adult worm surface proteins (17.5 kDa, 32 kDa, 42 kDa) but not microfilarial surface proteins.
    • Stage-specific carbohydrate moieties were found on adult worms and eggs, but not microfilariae.

    Conclusions:

    • The study successfully isolated Onchocerca volvulus microfilariae and characterized stage-specific surface proteins.
    • Surface proteins of adult worms, but not microfilariae, elicit a response in human infection serum.
    • These findings contribute to understanding parasite-host interactions and may aid in developing new control strategies.