A Protocol for co-Injecting Cells with Pulverized Fibers for Improved Cell Survival and Engraftment

Adipose tissue is crucial for medical applications such as tissue reconstruction, cosmetic procedures, and correcting soft tissue deformities. Significant advances in the use of adipose tissue have been achieved through Coleman's studies in fat grafting, which gained widespread acceptance due to its effectiveness and safety. Despite its benefits, adipose tissue grafting faces several limitations, including high absorption rates due to insufficient support or anchorage, replacement by fibrous tissue, migration from the intended site, and loss of the initial desired morphology post-administration. To counteract these constraints, there is a need for improved grafting techniques that enhance the predictability and consistency of outcomes. Biomaterials are extensively used in tissue engineering to support cell adhesion, proliferation, and growth. Both natural and synthetic materials have shown promise in creating suitable microenvironments for adipose tissue regeneration. PLGA, a synthetic copolymer, is particularly notable for its biocompatibility, biodegradability, and tunable mechanical properties. Here, we describe a protocol using milled electrospun poly(lactic-co-glycolic acid) (PLGA) fibers combined with lipoaspirated tissue to create a fibrous slurry for injection. By pulverizing PLGA fiber mats to create fiber fragments with increased pore size and porosity, we can influence key cellular responses and enhance the success of adipose tissue-grafting procedures. This approach improves anchorage and support for adipocytes, thereby increasing cell viability. This method aims to enhance vascularity, perfusion, and volume retention in adipose tissue grafts, which addresses many of the limitations of current approaches to adipose tissue grafting and holds promise for more consistent and successful outcomes. Key features • Adipose tissue for tissue reconstruction. • Need for improved engraftment and volume retention. • Pulverized PLGA fiber mats to create a fibrous "slurry" that allows injection. • PLGA fibers co-injected with lipoaspirated tissue. • Improved adipose engraftment outcomes (e.g., perfusion, vascularity, and retention of graft volume).