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Fixation and Sectioning

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...

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Related Experiment Video

Updated: Jun 6, 2025

Shrinkage of Dental Composite in Simulated Cavity Measured with Digital Image Correlation

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Published on: July 21, 2014

Surface Roughness and Color Stability of Newly Developed Resin Composites With Color Adjustment Potential After

G A Maghaireh¹, H Alzraikat², H K Alakhras³

¹*Ghada A Maghaireh, BDS, MS, ABOD, Department of Conservative Dentistry, Jordan University of Science and Technology, Irbid, Jordan.

Operative Dentistry

|November 27, 2024

Summary

Resin composites with color adjustment potential (CAP) showed varying color stability when exposed to staining solutions. Neo Spectra ST exhibited the best color stability, while Omnichroma and Charisma Diamond One showed the highest color change.

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Area of Science:

Dental Materials Science
Polymer Chemistry
Restorative Dentistry

Background:

Resin composites (RCs) are widely used in dental restorations.
Color adjustment potential (CAP) RCs aim to simplify shade matching.
Evaluating the long-term color stability of these materials is crucial for clinical success.

Purpose of the Study:

To compare the surface roughness and color stability of three CAP RCs (Omnichroma, Neo Spectra ST, Charisma Diamond One) against a conventional RC (Filtek Z350XT).
To assess the effect of common staining solutions (cola, coffee, black tea) on these properties over 21 days.

Main Methods:

Forty specimens of each RC material were prepared and divided into subgroups based on immersion solutions (cola, coffee, black tea, distilled water).
Surface roughness was measured using a non-contact optical profilometer at baseline and after 21 days.
Color change (ΔE00) was evaluated using a spectrophotometer at multiple time points (baseline, 1, 7, 21 days).

Main Results:

Filtek Z350XT exhibited significantly lower surface roughness compared to CAP RCs; no significant differences in roughness were found among CAP RCs.
No significant surface roughness changes occurred after immersion in staining solutions for any material.
Neo Spectra ST demonstrated the least color change, followed by Filtek Z350XT, while Omnichroma and Charisma Diamond One showed the most significant color alteration.

Conclusions:

While CAP RCs offer potential shade matching benefits, their color stability varies significantly when exposed to common beverages.
Neo Spectra ST appears to be the most color-stable among the tested CAP RCs.
Clinicians should consider the potential for color change with specific CAP RCs, particularly Omnichroma and Charisma Diamond One, in esthetic restorations.