Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fibroblast-derived matrix human skin equivalents reveal distinct roles for papillary and reticular fibroblasts in human skin barrier integrity and aging.

Scientific reports·2026
Same author

Mild psoriasis as a suitable model for proof-of-mechanism in a phase 1B setting: Results from a double-blind placebo-controlled trial with guselkumab.

British journal of clinical pharmacology·2025
Same author

Evaluation of PLGA, lipid-PLGA hybrid nanoparticles, and cationic pH-sensitive liposomes as tuberculosis vaccine delivery systems in a Mycobacterium tuberculosis challenge mouse model - A comparison.

International journal of pharmaceutics·2024
Same author

Lesional Psoriasis is Associated With Alterations in the Stratum Corneum Ceramide Profile and Concomitant Decreases in Barrier Function.

Experimental dermatology·2024
Same author

The phase behavior of skin-barrier lipids: A combined approach of experiments and simulations.

Biophysical journal·2024
Same author

Guselkumab treatment normalizes the stratum corneum ceramide profile and alleviates barrier dysfunction in psoriasis: results of a randomized controlled trial.

Journal of lipid research·2024

Related Experiment Video

Updated: Jun 6, 2025

Flash NanoPrecipitation for the Encapsulation of Hydrophobic and Hydrophilic Compounds in Polymeric Nanoparticles
10:12

Flash NanoPrecipitation for the Encapsulation of Hydrophobic and Hydrophilic Compounds in Polymeric Nanoparticles

Published on: January 7, 2019

22.0K

A Versatile, Low-Cost Modular Microfluidic System to Prepare Poly(Lactic-co-Glycolic Acid) Nanoparticles With

Malene Aaby Neustrup1,2, Tom H M Ottenhoff2, Wim Jiskoot1

  • 1Division of BioTherapeutics, Leiden Academic Center for Drug Research, Leiden University, Leiden, The Netherlands.

Pharmaceutical Research
|November 27, 2024
PubMed
Summary

A new, low-cost modular microfluidic system using capillaries was developed to create poly(D,L-lactic-co-glycolic acid) (PLGA) nanoparticles. This system enables reproducible protein encapsulation for drug and vaccine delivery.

Keywords:
PLGAco-flowmicrofluidicsnanoparticlesprotein encapsulation

More Related Videos

Rapid, Scalable Assembly and Loading of Bioactive Proteins and Immunostimulants into Diverse Synthetic Nanocarriers Via Flash Nanoprecipitation
06:57

Rapid, Scalable Assembly and Loading of Bioactive Proteins and Immunostimulants into Diverse Synthetic Nanocarriers Via Flash Nanoprecipitation

Published on: August 11, 2018

7.9K
Fabricating Highly Open Porous Microspheres HOPMs via Microfluidic Technology
05:21

Fabricating Highly Open Porous Microspheres HOPMs via Microfluidic Technology

Published on: May 16, 2022

2.8K

Related Experiment Videos

Last Updated: Jun 6, 2025

Flash NanoPrecipitation for the Encapsulation of Hydrophobic and Hydrophilic Compounds in Polymeric Nanoparticles
10:12

Flash NanoPrecipitation for the Encapsulation of Hydrophobic and Hydrophilic Compounds in Polymeric Nanoparticles

Published on: January 7, 2019

22.0K
Rapid, Scalable Assembly and Loading of Bioactive Proteins and Immunostimulants into Diverse Synthetic Nanocarriers Via Flash Nanoprecipitation
06:57

Rapid, Scalable Assembly and Loading of Bioactive Proteins and Immunostimulants into Diverse Synthetic Nanocarriers Via Flash Nanoprecipitation

Published on: August 11, 2018

7.9K
Fabricating Highly Open Porous Microspheres HOPMs via Microfluidic Technology
05:21

Fabricating Highly Open Porous Microspheres HOPMs via Microfluidic Technology

Published on: May 16, 2022

2.8K

Area of Science:

  • Nanotechnology
  • Materials Science
  • Biomedical Engineering

Background:

  • Microfluidics offers a promising route for nanoparticle preparation.
  • Existing chip-based microfluidic systems are often expensive and lack versatility.
  • There is a need for adaptable, cost-effective microfluidic solutions for nanoparticle synthesis.

Purpose of the Study:

  • To establish a modular microfluidic system using inexpensive capillaries and reusable components.
  • To prepare poly(D,L-lactic-co-glycolic acid) (PLGA) nanoparticles.
  • To incorporate water-soluble biomacromolecules, such as proteins, into PLGA nanoparticles.

Main Methods:

  • A two-syringe microfluidic system was employed to synthesize PLGA nanoparticles.
  • Parameters such as flow rates, solvents, and PLGA concentrations were systematically varied.
  • A three-syringe system was utilized to investigate protein incorporation into PLGA nanoparticles.

Main Results:

  • PLGA concentration influenced particle size (33-180 nm), while total flow rate affected nanoparticle diameter (197-77 nm).
  • Aqueous poly(vinyl alcohol) yielded neutral particles; ultrapure water or ethanol-water mixtures resulted in negatively charged particles.
  • Protein encapsulation efficiencies for ovalbumin and lysozyme exceeded 40% using the three-syringe system.

Conclusions:

  • A cost-effective and adaptable modular microfluidic system was successfully developed.
  • The system produces PLGA nanoparticles with highly reproducible diameters.
  • This technology is suitable for effective protein loading for advanced drug and vaccine delivery applications.