Related Experiment Video
Updated: Jun 6, 2025

Colorimetric Paper-based Detection of Escherichia coli, Salmonella spp., and Listeria monocytogenes from Large Volumes of Agricultural Water
Published on: June 9, 2014
Chemically modified filter paper with Jaman fruit extract for visual sensing of milk freshness
Muhammad Naeem Shabbir1, Adeel Ahmad2, Muhammad Aslam1
1Department of Chemistry, Division of Science and Technology, University of Education, Lahore 54770, Pakistan.
Abstract:
Developing a non-toxic, cost-effective, and user-friendly indicator for detecting milk freshness and spoilage, with potential applications in smart packaging, presents a significant challenge. In this study, we extracted natural anthocyanins (ACNs) from Jaman (Syzgium cumini) and incorporated it in to filter paper. The presence of ACNs in the extract was confirmed through UPLC-MS/MS analysis. To enhance stability and prevent leaching, the ACNs-loaded filter paper was coated with 1 % chitosan (CH). The sensor strips were thoroughly characterized using FTIR, contact angle measurements, XRD, and FE-SEM. These strips displayed a distinct and quantifiable color change from red to colorless to yellow across different pH levels. Additionally, in real-time milk spoilage detection, the sensor transitioned from light purple to colorless. The strips demonstrated reproducibility and stability, even under high temperatures and extreme pH conditions. These findings suggest that the ACNs-loaded sensor strips hold promise for use as smart packaging materials.

