Bacillus amyloliquefaciens modulate autophagy pathways to control Rhizoctonia solani infection in rice

The necrotrophic fungus Rhizoctonia solani significantly threatens rice harvests and agricultural productivity by causing sheath blight disease. This study investigates the potential of the plant growth-promoting rhizobacteria Bacillus amyloliquefaciens (SN13) as a biocontrol agent in the sensitive rice variety Swarna against R. solani infection. Disease incidence analysis reveals untreated rice plants suffer from R. solani infection, while SN13 treatment effectively suppresses fungal growth. In detached leaf assays, SN13 mitigates R. solani-induced damage, and physio-biochemical analyses indicate improved growth in SN13-treated rice plants. Notably, treatment with chloroquine, an autophagy inhibitor, increases disease incidence, whereas SN13 treatment enhances the formation of autophagosomes stained with Mono Dansyl Cadaverine (MDC) dye, as observed through confocal microscopy, suggesting the involvement of autophagy in plant defense against R. solani. Gene expression analysis reveals alterations in ATG and defence-related genes (BZ1, 5H5, and 8A1), affirming that SN13 activates autophagy and bolsters plant resilience. Metabolite analysis using GC-MS indicates the accumulation of defence signalling molecules such as gluconic acid, arabitol, glucopyranoside, ribose, xylopyranose, and arabinofuranoside. Overall, this study demonstrates the role of SN13 in inducing the autophagy response and modulating crucial defense pathways to control R. solani infection in rice var Swarna.