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Updated: Jun 6, 2025
Monitoring PD-1-Blocking Antibodies Bound to T Cells Derived from a Drop of Peripheral Blood
Published on: February 5, 2020
Discovery of an theranostic functional mAb for visualizing the sensitivity and effectiveness of PD-L1 checkpoint
Jin Wang1, Yanchen Li2, Junyu Zhang2
1Department of Pharmacy, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China; School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an 710061, China.
Abstract:
To address the challenges of inconsistent efficacy, low sensitivity, and subjective screening methods faced by PD-L1-targeted immune checkpoint therapies in the clinic. We propose to construct a therapeutic functional monoclonal antibody with tracking capability using tetrazolium-based bioorthogonal reaction turn-on fluorescence technology, focusing on the molecular biomarker PD-L1 for easy visualisation of therapeutic response. This therapeutic monoclonal antibody enables bioorthogonal reaction turn-on fluorescence to monitoring of tumors with varying degrees of PD-L1 expression, while preserving the activity of the PDL1 monoclonal antibody and improve the immune activation rate and achieve efficient anti-tumor effects. In conclusion, our proposed family of bio-orthogonal reaction-driven fluorescence turn-on methods can be used to identify therapeutic bifunctional monoclonal antibodies to PD-L1, which can be employed alongside the screening of PD-L1 antibodies for therapeutic effects. Abbreviations: CRT, Calreticulin; DCs, Dendritic cells; ELISA, Enzyme-linked immunosorbent assay; IFN-γ, Interferon-γ; IL-2, Interleukin-2; IHC, Immunohistochemistry; WB, Western blot.
