Search research articles

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Regenerable Biocidal Surfaces Enabled by Self-Assembled Block Copolymer Polyelectrolytes.

ACS applied materials & interfaces·2026

Same author

Degradable Cationic Polyesters with Tunable Anion-Induced Upper Critical Solution Temperature Coacervation.

Biomacromolecules·2026

Same author

Investigating the Role of Miscibility in Hydrogenated Dicyclopentadiene Resin/Polymer Blends: A Molecular Dynamics Study.

Polymers·2026

Same author

Mixed Matrix Membranes Composed of Graphene-Based Derivatives as Additives in PVAm for CO<sub>2</sub> Capture.

ACS applied engineering materials·2025

Same author

Investigating the Effects of pH and Temperature on the Properties of Lysozyme-Polyacrylic Acid Complexes via Molecular Simulations.

ACS omega·2025

Same author

Complexation and Thermal Stabilization of Protein-Polyelectrolyte Systems via Experiments and Molecular Simulations: The Poly(acrylic acid)/Lysozyme Case.

Polymers·2025

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 6, 2025

DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering

DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering

Published on: November 9, 2017

Interfacial interactions between DNA and polysaccharide-coated magnetic nanoparticles: Insight from simulations and

Maria Psarrou¹, Maria Vamvakaki¹, Kostas Karatasos²

¹Department of Materials Science and Technology, University of Crete, Heraklion, Crete 700 13, Greece; Institute of Electronic Structure and Laser, FORTH, Heraklion, Crete 700 13, Greece.

Colloids and Surfaces. B, Biointerfaces

|November 27, 2024

Summary

Dextran-coated magnetic nanoparticles (MNPs) effectively bind DNA for gene delivery. Optimizing nanoparticle coatings and salt concentration enhances DNA complexation and binding stability, crucial for effective delivery.

Keywords:

Gene delivery Interfacial interactions Magnetic nanoparticles Molecular dynamics simulations Polymer coated particles Polysaccharides

More Related Videos

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures

Published on: January 19, 2019

DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers

DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers

Published on: October 25, 2017

Related Experiment Videos

Last Updated: Jun 6, 2025

DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering

DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering

Published on: November 9, 2017

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures

Published on: January 19, 2019

DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers

DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers

Published on: October 25, 2017

Area of Science:

Biomaterials Science
Nanotechnology
Molecular Biophysics

Background:

Magnetic nanoparticles (MNPs) are promising for gene delivery.
Dextran coating is explored to improve MNP-DNA interactions.
Understanding interfacial forces is key for optimizing DNA delivery systems.

Purpose of the Study:

To investigate the structural and energetic stability of dextran-coated MNPs interacting with DNA.
To elucidate the mechanisms of Dextran adsorption and DNA-Dextran complexation.
To determine the influence of ionic strength on DNA-MNP interactions for gene delivery.

Main Methods:

All-atom Molecular Dynamics (MD) simulations to analyze atomic-level interactions.
Examination of Coulombic and hydrogen bonding interactions.
Comparison of coated versus uncoated MNPs.
Complementary experimental validation at larger length scales.

Main Results:

Dextran adsorption onto MNPs is primarily driven by Coulombic interactions, with hydrogen bonding enhancing layer integrity.
Dextran-DNA complexation is mediated by electrostatic interactions.
Increased salt concentration promotes DNA complexation by modifying Coulombic forces and enhancing hydrogen bonding between Dextran and DNA.
The dextran interfacial layer significantly enhances DNA association with the magnetic surface.

Conclusions:

Tailoring nanoparticle coatings and ionic strength are critical for optimizing DNA delivery via MNPs.
Favorable interfacial forces and DNA/MNP binding stability can be fine-tuned for improved gene delivery efficiency.
The study provides atomic-level insights into MNP-DNA interactions relevant to physiological conditions.