One-step construction of bioinspired multi-enzyme mimicking nanozyme as a universal platform for multi-mode sensing...

Nanozymes, a category of nanomaterials with exceptional enzyme-like activity, exhibit the significant promise to overcome the inherent limitations of natural enzymes. Inspired by the active site structure of natural laccase, a biomimetic MA-Cu nanozyme with three-dimensional network structure was constructed in water system through one-step complexation based on the specific coordination between nitrogen-rich triazine heterocyclic melamine and Cu²⁺, in a facile, green and economical manner. Compared to natural laccase, MA-Cu possesses superior multi-enzyme mimicking activity, stability and cost-effectiveness. Through comprehensive characterizations, activity tests and theoretical calculations, the catalytic mechanism and the ligand-tunability of enzyme-like activity have been thoroughly investigated. Based on its multi-enzyme-like activities, a multifunctional monitoring platform for sulfide in food, epinephrine in preparations and glutathione in cells was successfully constructed, respectively. Notably, a green degradation and discrimination platform based on MA-Cu for various pollutants was developed, exhibiting distinguished substrate universality and detoxication capacity. As a stable, easily scalable and commercially applicable nanozyme, MA-Cu is expected to become a compelling candidate for replacing natural enzyme, showing excellent prospects in environmental remediation and biosensing.