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UPLC-MS/MS-based serum metabolomics analysis for comprehensive pathological myopia profiling.

Xin Liu1, Yue Wu1, Yuying Liu1

  • 1Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, No. 100 Haining Road, Shanghai, 200080, China; National Clinical Research Center for Eye Diseases, Shanghai, China; Shanghai Key Laboratory of Fundus Disease, Shanghai, China; Shanghai Engineering Center for Visual Science and Photomedicine, Shanghai, China; Shanghai Engineering Center for Precise Diagnosis and Treatment of Eye Diseases, Shanghai, China.

Experimental Eye Research
|November 27, 2024
PubMed
Summary
This summary is machine-generated.

Pathological myopia (PM) involves distinct metabolic differences compared to high myopia (HM). Identifying these metabolic alterations, such as 4-hydroxy-l-glutamic acid levels, offers insights into PM

Keywords:
Glutamic acidLipid metabolismMetabolomicsPathological myopiaProstaglandinSuccinic acidUPLC‒MS/MS

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Area of Science:

  • Ophthalmology
  • Metabolomics
  • Biochemistry

Background:

  • Pathological myopia (PM) is linked to blindness-causing ocular morbidities.
  • PM and high myopia (HM) are distinct conditions, but their differences remain unclear.
  • Understanding PM pathogenesis is crucial for preventing vision loss.

Purpose of the Study:

  • To comprehensively identify metabolic alterations in pathological myopia (PM).
  • To differentiate metabolic profiles between PM, high myopia (HM), and low myopia (LM).
  • To explore the role of specific metabolites and pathways in PM development.

Main Methods:

  • Ultra-performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) was used.
  • Comparative analysis of metabolite profiles across three groups: PM, HM, and LM.
  • Venn diagrams and receiver operating characteristic (ROC) curve analysis were employed.

Main Results:

  • 134, 125, and 81 differential metabolites were identified across comparisons.
  • 32 differential metabolites overlapped between PM vs. HM and PM vs. LM.
  • Key risk factors identified include high 4-hydroxy-l-glutamic acid and low succinic semialdehyde; alanine, aspartate, and glutamate metabolism was significantly altered.

Conclusions:

  • Significant metabolic differences exist between PM and HM patients.
  • Metabolic alterations, particularly in amino acid metabolism, are associated with PM.
  • These findings offer novel insights into the molecular mechanisms underlying pathological myopia.