Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Structural basis of multimodal adsorption and infection initiation by <i>Vibrio</i> phage Peru-2.

bioRxiv : the preprint server for biology·2026

Same author

Point-of-Care Ultrasound After Non-fatal Drowning in Rural Western Nepal: A Case Report.

Clinical practice and cases in emergency medicine·2026

Same author

Acceptability, appropriateness, and feasibility of a point-of-care ultrasound program for the diagnosis of forearm fractures in rural Nepal: a mid-level provider qualitative study.

BMC medical education·2026

Same author

High-resolution mapping of CCR4-NOT recruitment elements reveals transcriptome-wide drivers of mRNA decay.

Cell reports·2026

Same author

Prognostic Model-Guided Randomization Improves Efficiency in Early-Phase Trials: Evidence From Surveys and Simulations.

Clinical and translational science·2026

Same author

Genome-wide profiling of RNA 2'-O-methylation in neurons and identification of orphan snoRNA targets.

Research square·2026

Same journal

A human-specific genetic modifier reconfigures large-scale cortical network dynamics underlying behavioral performance.

bioRxiv : the preprint server for biology·2026

Same journal

<i>Staphylococcus aureus</i> uses a eukaryotic-like uridyltransferase to make UDP-GlcNAc for cell wall synthesis.

bioRxiv : the preprint server for biology·2026

Same journal

Dynamic redistribution of eIF4F controls cap-dependent translation initiation.

bioRxiv : the preprint server for biology·2026

Same journal

When does additional information improve accuracy of RNA secondary structure prediction?

bioRxiv : the preprint server for biology·2026

Same journal

Normative brain-state trajectories reveal deviation from healthy aging in Alzheimer's disease.

bioRxiv : the preprint server for biology·2026

Same journal

Noradrenergic infraslow rhythm during sleep is the critical link between heart-rate dynamics and memory consolidation.

bioRxiv : the preprint server for biology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 6, 2025

DNAzyme-dependent Analysis of rRNA 2’-O-Methylation

DNAzyme-dependent Analysis of rRNA 2’-O-Methylation

Published on: September 16, 2019

Generating snoRNA-guided Programmable 2'- O -methylation.

Justin Zhao, Eric Cockman, Xinhe Yin

Biorxiv : the Preprint Server for Biology

|November 28, 2024

Summary

This summary is machine-generated.

This study introduces a new assay to validate 2'-O-methylation (Nm) sites, crucial for RNA function. Synthetic small nucleolar RNAs (snoRNAs) can guide these modifications, impacting gene expression and translation efficiency.

More Related Videos

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

Published on: July 28, 2017

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

Related Experiment Videos

Last Updated: Jun 6, 2025

DNAzyme-dependent Analysis of rRNA 2’-O-Methylation

DNAzyme-dependent Analysis of rRNA 2’-O-Methylation

Published on: September 16, 2019

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

Published on: July 28, 2017

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

Area of Science:

Molecular Biology
RNA Biology
Epigenetics

Background:

Small nucleolar RNAs (snoRNAs) guide 2 -O-methylation (Nm) modifications essential for RNA processing.
Current methods for mapping Nm sites, particularly on messenger RNAs (mRNAs), lack validation and consensus.
A validated technique is needed to advance the study of Nm modifications and their roles in gene expression.

Purpose of the Study:

To develop and validate a novel assay for quantifying 2 -O-methylation (Nm) at single nucleotide resolution.
To investigate the potential of synthetic snoRNAs in guiding Nm modifications.
To explore the impact of Nm modifications on gene expression, including mRNA abundance and translation efficiency.

Main Methods:

Development and application of the RNase H-based Nm-VAQ assay for Nm quantification across RNA species.
Optimization of the assay for low-abundance transcripts like mRNAs.
Design and utilization of synthetic snoRNAs to guide Nm modifications in genetic knockout models and reporter assays.

Main Results:

The Nm-VAQ assay successfully quantifies Nm modifications at single nucleotide resolution in rRNA, snRNA, and mRNA.
Optimized assay enables study of Nm in low-abundance transcripts.
Synthetic snoRNAs can be engineered to guide Nm to specific sites, rescue modifications in knockout models, and influence reporter gene expression (luciferase).

Conclusions:

The RNase H-based Nm-VAQ assay provides a crucial validation tool for Nm mapping.
Synthetic snoRNAs offer a powerful platform for studying and manipulating Nm modifications.
Nm modifications, guided by snoRNAs, significantly impact gene expression by affecting mRNA abundance and translation efficiency.