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Renin precursor synthesis and renin-binding proteins in the mouse
Clinical Science (London, England : 1979)
|December 1, 1979
Summary
Mouse studies reveal renin is synthesized as a precursor (pre-prorenin) and stored as active renin (40,000 MW) in kidneys and glands. Plasma contains active renin and high-molecular-weight forms, possibly complexes with inhibitors.
Area of Science:
- Biochemistry
- Molecular Biology
- Physiology
Background:
- Renin is a key enzyme in the renin-angiotensin system, regulating blood pressure.
- Understanding renin's molecular forms and interactions is crucial for physiological studies.
Purpose of the Study:
- To investigate the precursor synthesis, storage, and plasma forms of renin in mice.
- To characterize the molecular nature and interactions of different renin forms.
Main Methods:
- Studied precursor synthesis and molecular weights of renin forms.
- Analyzed renin storage in kidney and submaxillary gland.
- Investigated binding of purified renin to plasma proteins using 125I-labeling.
Main Results:
- Renin is synthesized as pre-prorenin (50,000 MW) and stored as active renin (40,000 MW).
- Plasma predominantly contains active 40,000 MW renin, with high-molecular-weight forms (800,000 and 70,000 MW) also present.
- Active renin binds to specific plasma protease inhibitors (alpha 2-macroglobulin, inter-alpha-trypsin inhibitor, alpha2-antithrombin), lipoprotein, and an unidentified protein.
Conclusions:
- High-molecular-weight renin forms in plasma likely represent complexes with inhibitors and lipoproteins.
- This study elucidates the molecular heterogeneity of renin and its plasma interactions.