From Hypoxia to Buoyancy: Serotonin and Oxygen Deprivation Rewire Neutrophils

Herein, we describe a method to purify higher buoyancy neutrophils in vitro after exposure of whole blood to brief hypoxia and reoxygenation in combination with platelet-derived serotonin. These higher buoyancy neutrophils display enhanced ability to form neutrophil extracellular traps and increment the tryptamine-protein adducts. Similar changes are identified in neutrophils isolated from patients with systemic lupus erythematosus. These results suggest that neutrophils may be rewired in tissues under conditions of hypoxia-reperfusion such as those seen in chronic inflammatory conditions.