Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

On the structure of poly d(A-S4T).

W Bökemeier, A G Lezius

    Nucleic Acids Research
    |March 11, 1986
    PubMed
    Summary
    This summary is machine-generated.

    The helical twist of poly d(A-s4T) DNA was found to be 14 base pairs per turn. This study reveals a unique mononucleotide structure, differing from typical B DNA, due to base pair tilting and propeller twist.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    N-terminal variants of fatty acid-binding protein from bovine heart overexpressed in Escherichia coli.

    Journal of biotechnology·1994
    Same author

    Isoforms of fatty-acid-binding protein in bovine heart are coded by distinct mRNA.

    European journal of biochemistry·1993
    Same author

    Cloning of a full-length complementary DNA for fatty-acid-binding protein from bovine heart.

    European journal of biochemistry·1988
    Same author

    Base pairing structure in the poly d(G-T) double helix: wobble base pairs.

    Nucleic acids research·1978
    Same author

    An ATPase depending on the presence of single-stranded DNA from mouse myeloma.

    European journal of biochemistry·1976
    Same author

    Rapid isolation of highly active RNA polymerase from Escherichia coli and its subunits by matrix-bound heparin.

    European journal of biochemistry·1975

    Area of Science:

    • Biochemistry
    • Structural Biology
    • Nucleic Acid Chemistry

    Background:

    • Polydeoxynucleotides exhibit diverse helical structures influencing their biological functions.
    • Understanding DNA conformation is crucial for molecular biology and drug design.

    Purpose of the Study:

    • To determine the helical twist and structural characteristics of poly d(A-s4T).
    • To investigate the discrepancy between NMR and X-ray diffraction data for this DNA analog.

    Main Methods:

    • Analysis of cleavage patterns of poly d(A-s4T) adsorbed on calcium phosphate.
    • 31P Nuclear Magnetic Resonance (NMR) spectroscopy.
    • X-ray fiber diffraction analysis (for comparison).

    Main Results:

    Related Experiment Videos

    • The helical twist of poly d(A-s4T) was determined to be 14 base pairs (bp) per turn.
    • Cleavage patterns and 31P NMR spectra indicated a mononucleotide structure, not an alternating B DNA.
    • Failure of nucleosome formation further excluded a B-type structure.
    • Discrepancy between NMR and X-ray data explained by base pair tilting (base roll) and propeller twist.

    Conclusions:

    • Poly d(A-s4T) adopts a unique helical structure distinct from canonical B DNA.
    • Base roll and propeller twist are key factors in its structural conformation and stability.
    • Interstrand stacking of 4-thiothymidines contributes significantly to helical stability.