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Development of a Platform for High-Resolution Ion Mobility Separations Coupled with Messenger Tagging Infrared
Christopher P Harrilal1, Sandilya V B Garimella1, Randolph V Norheim1
1Biological Sciences Division, Pacific Northwest National Laboratory, P.O. Box 999, Richland, Washington 99354, United States.
Analytical Chemistry
|November 28, 2024
Summary
A new analytical platform combines high-resolution ion mobility and cryogenic vibrational spectroscopy for precise compound identification. This enables confident structural characterization and differentiation of similar ions through orthogonal measurements.
Area of Science:
- Analytical Chemistry
- Spectroscopy
- Physical Chemistry
Background:
- Accurate compound identification necessitates precise and orthogonal analytical measurements.
- Distinguishing between isomeric and isobaric ions remains a significant analytical challenge.
- Existing methods may lack the sensitivity or resolution for complex structural characterizations.
Purpose of the Study:
- To develop and characterize a novel analytical platform integrating high-resolution ion mobility and cryogenic vibrational spectroscopy.
- To achieve high-precision structural characterization of ions, enabling confident identification of similar compounds.
- To demonstrate the utility of the platform for both targeted and untargeted analytical approaches.
Main Methods:
- Integration of a Structures for Lossless Ion Manipulations Ion Mobility (SLIM IM) device with messenger tagging infrared spectroscopy.
- Utilizing a cryogenically held (30 K) TW-SLIM module within a qTOF mass spectrometer for collisional cooling and N2 complex formation.
- Operation in two modes: Ion Mobility Spectrometry + Infrared (IMS+IR) for mobility-selected ions and IR-only mode for all mass-resolved ions.
Main Results:
- The platform enables temporal separation of isomeric/isobaric ions and sensitive gas-phase structural descriptions.
- Orthogonal measurements provide precise descriptors for resolving and identifying highly similar ions.
- Demonstrated high-resolution ion mobility separations and simultaneous IR spectral recording of all ions.
Conclusions:
- The developed analytical platform offers a powerful tool for high-precision structural characterization and compound identification.
- The integration of SLIM IM and cryogenic vibrational spectroscopy provides unique benefits for resolving complex molecular structures.
- Initial benchmarking confirms the instrument's utility for advanced analytical applications.
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