Hsp70-Hsp90 organising protein (HOP/STIP1) is required for KSHV lytic replication
Elisa Kirigin1, Michael Obinna Okpara1, Lorraine Matandirotya1,2
1Biomedical Biotechnology Research Unit (BioBRU), Department of Biochemistry and Microbiology, Rhodes University, Makhanda, 6139, South Africa.
The Journal of General Virology
|November 28, 2024
Summary
Hsp90-Hsp70-organizing protein (HOP) is crucial for Kaposi
Area of Science:
- Molecular Virology
- Cancer Biology
- Cellular Proteostasis
Background:
- Kaposi's sarcoma-associated herpesvirus (KSHV) causes endothelial cancers.
- KSHV relies on host molecular chaperones Hsp70 and Hsp90 for protein folding during infection.
- Co-chaperones, such as Hsp90-Hsp70-organizing protein (HOP), regulate chaperone function but HOP's role in KSHV is unknown.
Purpose of the Study:
- To investigate the role of Hsp90-Hsp70-organizing protein (HOP) in Kaposi's sarcoma-associated herpesvirus (KSHV) infection.
- To determine if HOP is a host factor required for KSHV replication.
Main Methods:
- Studied the interaction between HOP, Hsp70, and Hsp90 in the context of KSHV.
- Assessed the impact of HOP on KSHV lytic replication in primary effusion cell lines.
Main Results:
- Demonstrated a novel function for HOP in KSHV biology.
- Identified HOP as a necessary host factor for effective lytic replication of KSHV.
- Confirmed HOP's interaction with Hsp70 and Hsp90 chaperone systems.
Conclusions:
- Hsp90-Hsp70-organizing protein (HOP) is a newly identified host factor essential for KSHV lytic replication.
- This finding expands our understanding of host-pathogen interactions in KSHV pathogenesis and suggests potential therapeutic targets.
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