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Automatic detection of multi-antibiotic residues simultaneously in aquatic products by visual protein microarray
Minmin Tang1, Wei Zhou2, Jinzhu Song2
1Key Laboratory of Food Contact Materials Safety, State Administration for Market Regulation, Jiangsu Product Quality Testing & Inspection Institute, Nanjing, Jiangsu 210007, China; State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.
A novel protein microarray chip enables high-throughput, simultaneous detection of three antibiotic families in 96 samples within an hour. This automated system offers a sensitive and reliable method for monitoring antibiotic residues in aquatic products.
Area of Science:
- Analytical Chemistry
- Biotechnology
- Food Safety
Background:
- Antibiotic residues in food pose significant health risks.
- Current detection methods can be time-consuming and lack multiplexing capabilities.
Purpose of the Study:
- To develop a novel, high-throughput protein microarray chip for simultaneous detection of multiple antibiotic residues.
- To validate the chip's performance in various aquatic products.
Main Methods:
- Development of a 96-well microplate-based protein microarray chip.
- Automated biochip analyzer for high-throughput sample processing (96 samples/60 min).
- Visual and quantitative analysis using color depth and scanner.
Main Results:
- Simultaneous detection of nitrofuran metabolites (NFMs), chloramphenicol (CAP), and fluoroquinolones (FQs).
- Achieved limits of detection (LODs) as low as 0.07 μg/kg for CAP.
- High recovery rates (89.4%–111.2%) in crucian carp, shrimp, and scallops.
Conclusions:
- The developed protein microarray chip provides a rapid, sensitive, and multiplexed solution for antibiotic residue detection.
- This technology holds significant potential for routine food safety supervision and quality control.
