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MiR-15a-5p Knockdown up-Regulated ABCB1 Expression and Abated HNSCC Progression via the NF-κB Signaling Pathway

  • 0Outpatient Department, Renmin Hospital of Wuhan University, Wuhan, China.
Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research +

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November 28, 2024

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November 28, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

In head and neck squamous cell carcinoma (HNSCC), inhibiting miR-15a-5p increases ABCB1 levels, suppressing tumor progression via the NF-κB pathway. These findings identify ABCB1 and miR-15a-5p as potential HNSCC biomarkers.

Area Of Science

  • Oncology
  • Molecular Biology
  • Biochemistry

Background

  • Head and neck squamous cell carcinoma (HNSCC) is characterized by high invasion and heterogeneity, leading to poor clinical outcomes.
  • Reliable biomarkers are crucial for improving HNSCC diagnosis and treatment strategies.

Purpose Of The Study

  • To identify novel molecular biomarkers for head and neck squamous cell carcinoma (HNSCC).
  • To investigate the regulatory role of microRNAs (miRNAs) and their target genes in HNSCC progression.

Main Methods

  • Screening of frequently mutated genes using the cBioPortal database.
  • miRNA-mRNA co-expression analysis to identify interactive miRNAs.
  • In vitro assays (CCK-8, Transwell) to assess cell proliferation, migration, and invasion.
  • In vivo studies using a xenograft mouse model to evaluate therapeutic efficacy.

Main Results

  • ATP binding cassette transporter 1 (ABCB1) exhibited the highest mutation frequency in HNSCC; decreased ABCB1 correlated with better patient prognosis.
  • MiR-15a-5p was identified as a regulator of ABCB1. Inhibition of miR-15a-5p led to ABCB1 upregulation.
  • Knockdown of miR-15a-5p significantly suppressed HNSCC cell proliferation, migration, and invasion in vitro and reduced tumor growth and metastasis in vivo, potentially via the NF-κB signaling pathway.

Conclusions

  • MiR-15a-5p knockdown enhances ABCB1 expression, thereby inhibiting HNSCC progression through the NF-κB signaling pathway.
  • ABCB1 and miR-15a-5p show potential as predictive biomarkers for therapeutic interventions in HNSCC.

Keywords:

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