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The Tumor Microenvironment

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...

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Updated: Jun 6, 2025

Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors

Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors

Published on: June 7, 2018

Mutation-Driven Immune Microenvironments in Non-Small Cell Lung Cancer: Unrevealing Patterns through Cluster

Youngtaek Kim¹, Joon Yeon Hwang¹, Kwangmin Na¹

¹Department of Research Support, Yonsei Biomedical Research Institute, Yonsei University College of Medicine, Seoul, Korea.

Yonsei Medical Journal

|November 28, 2024

Summary

This summary is machine-generated.

This study reveals distinct immune microenvironments in non-small cell lung cancer (NSCLC) based on specific oncogene mutations. Understanding these differences can guide personalized cancer treatments.

Keywords:

EGFR gene Non-small cell lung cancer ScRNA-seq TP53 gene mutation

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Last Updated: Jun 6, 2025

Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors

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Predictive Immune Modeling of Solid Tumors

Predictive Immune Modeling of Solid Tumors

Published on: February 25, 2020

Area of Science:

Oncology
Immunology
Genetics

Background:

Non-small cell lung cancer (NSCLC) is a leading cause of cancer death.
Tumor heterogeneity significantly impacts treatment response.
Single-cell RNA sequencing (scRNA-seq) offers high-resolution insights into complex biological systems.

Purpose of the Study:

To comprehensively analyze the tumor microenvironment (TME) at the single-cell level.
To identify tumor heterogeneity driven by major oncogene mutations in NSCLC.
To correlate specific mutations with immune cell and stromal components.

Main Methods:

Utilized scRNA-seq data from 64,369 primary tumor cells across 21 NSCLC patients.
Focused on mutations in EGFR, ALK, BRAF, KRAS, TP53, and wild-type.
Performed detailed analysis of immune cell and stromal components within the TME.

Main Results:

Identified two distinct mutation clusters (MC1: KRAS, TP53, EGFR+TP53; MC2: EGFR, BRAF, ALK) with differential immune responses.
MC1 exhibited higher tertiary lymphoid structures and specific immune cell populations (T/NK, B cells).
MC2 showed enrichment of inflammatory cytokines (TNF, IL1B) and alternative immune pathways. EGFR+TP53 mutations displayed distinct cellular activity and differentiation profiles compared to EGFR mutations.

Conclusions:

Mutation type is closely associated with the tumor microenvironment in NSCLC.
These findings provide a basis for developing personalized diagnostic and therapeutic strategies.
Single-cell analysis reveals critical TME differences linked to specific oncogenic drivers.