Concordance between immunohistochemistry and MSI analysis for detection of MMR/MSI status in colorectal cancer patients
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View abstract on PubMed
Summary
This summary is machine-generated.Screening colorectal cancer (CRC) patients for mismatch repair/microsatellite instability (MMR/MSI) status is crucial. Immunohistochemistry (IHC) and MSI analysis show perfect concordance, supporting universal MMR/MSI evaluation in CRC patients.
Area Of Science
- Oncology
- Molecular Biology
- Genetics
Background
- MMR/MSI status screening in colorectal cancer (CRC) is standard practice.
- MMR/MSI status has predictive and prognostic roles in CRC.
- MMR/MSI screening aids in identifying Lynch Syndrome (LS).
Purpose Of The Study
- To evaluate the concordance between immunohistochemistry (IHC) and MSI analysis for MMR/MSI status detection in CRC patients.
- To assess the reliability of IHC and MSI analysis in a specific population (Kuantan, Pahang).
Main Methods
- Fifty selected CRC cases previously classified by IHC as deficient mismatch repair (dMMR) or proficient mismatch repair (pMMR) were analyzed.
- Microsatellite Instability (MSI) analysis was performed using the MSI Analysis System 1.2 (Promega).
Main Results
- MSI analysis revealed MSI-High in 26%, MSI-Low in 6%, and Microsatellite Stable in 68% of cases.
- A perfect concordance (Kappa value 0.896) was observed between MSI analysis and IHC methods.
- Only 4% discordance was noted, with MSI analysis correctly identifying all but one dMMR case identified by IHC.
Conclusions
- Findings support the routine evaluation of MMR/MSI status in all newly diagnosed CRC patients.
- The choice between IHC and MSI analysis depends on available expertise and equipment.
- IHC is a highly valuable method due to its feasibility and reproducibility.
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