Related Experiment Video
Updated: Jun 6, 2025
12:15
In Vitro Polymerization of F-actin on Early Endosomes
Published on: August 28, 2017
9.0K
Precise Regulations at the Subcellular Level through Intracellular Polymerization, Assembly, and Transformation.
Le He1,2, Fanying Meng3, Ran Chen3
1School of Materials Science and Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.
JACS Au
|November 29, 2024
Summary
Intracellular synthesis strategies like polymerization and self-assembly offer new ways to target cell functions. These methods show promise for drug delivery, cancer treatment, and modulating cell metabolism.
Area of Science:
- Biochemistry
- Cell Biology
- Nanotechnology
Background:
- Living cells utilize subregions for efficient function.
- Subcellular dysfunction is a key target for drug design and therapy.
- Intracellular synthesis strategies offer advantages in drug delivery and targeting.
Purpose of the Study:
- To review recent advancements in intracellular synthesis for precise cellular control.
- To explore applications in cancer diagnosis, treatment, and cellular state modulation.
- To discuss challenges and future opportunities in intracellular functional regulation.
Main Methods:
- Review of endogenous and exogenous stimuli-triggered intracellular polymerization.
- Analysis of self-assembly and dynamic morphology transformation of biomolecules.
- Exploration of strategies for subcellular level control.
Main Results:
- Intracellular polymerization and self-assembly enable controlled drug delivery and targeting.
- These strategies enhance pharmacokinetic profiles and reduce drug resistance.
- Applications demonstrated in cancer therapy and metabolic regulation.
Conclusions:
- Intracellular synthesis represents a powerful approach for cellular function regulation.
- Significant progress has been made in applying these strategies to disease treatment.
- Future research should focus on overcoming challenges in intracellular reaction control.
More Related Videos
10:27Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells
Published on: March 9, 2012
10.8K
10:01Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro
Published on: April 8, 2020
5.8K
Related Concept Videos
01:23Regulation of Expression at Multiple Steps
872
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
872
02:24Regulation of Expression Occurs at Multiple Steps
22.5K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
22.5K
02:22Regulated mRNA Transport
6.2K
In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
6.2K
01:13Actin Polymerization and Cell Motility
5.1K
Actin is a family of globular proteins that are highly abundant in eukaryotic cells. It makes up approximately 1-5% of total cell protein concentration. Actin monomers polymerize to form a complex network of polarized filaments, the actin cytoskeleton, that plays a crucial role in many cellular processes, including cell motility, division, endocytosis, and metastasis of cancer cells.
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate....
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate....
5.1K
02:11Proteins: From Genes to Degradation
12.0K
Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick. Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation.
Transcription is the synthesis of RNA...
Transcription is the synthesis of RNA...
12.0K
01:45Regulation of Nuclear Protein Sorting
2.4K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.4K