Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Endoglin is not a mediator in 7-ketocholesterol-induced endothelial activation in liver sinusoidal endothelial cells in vitro.

Inflammation research : official journal of the European Histamine Research Society ... [et al.]·2026
Same author

Identifying Structural Factors Governing the Photodynamic Activity of Phthalocyanines.

Journal of medicinal chemistry·2026
Same author

Promising Protocol for In Vivo Experiments with Betulin.

Pharmaceutics·2025
Same author

Discovery of PXR Antagonist MI891 and PXR Degrader MI1013 and Their Roles in Hepatic Gene Regulation.

Journal of medicinal chemistry·2025
Same author

Dimethyl fumarate attenuates bile acid retention and liver fibrosis in a mouse model of cholestasis.

American journal of physiology. Gastrointestinal and liver physiology·2025
Same author

The hypolipidemic effect of MI-883, the combined CAR agonist/ PXR antagonist, in diet-induced hypercholesterolemia model.

Nature communications·2025

Related Experiment Video

Updated: Jun 6, 2025

Targeting Drugs to Larval Zebrafish Macrophages by Injecting Drug-Loaded Liposomes
11:31

Targeting Drugs to Larval Zebrafish Macrophages by Injecting Drug-Loaded Liposomes

Published on: February 18, 2020

7.7K

Dexamethasone Acetate-Loaded PLGA Nanospheres Targeting Liver Macrophages.

Barbora Boltnarova1, Anna Durinova2, Lenka Jandova3

  • 1Department of Pharmaceutical Technology, Faculty of Pharmacy in Hradec Kralove, Charles University, Akademika Heyrovskeho 1203, Hradec Kralove, 50005, Czech Republic.

Macromolecular Bioscience
|November 29, 2024
PubMed
Summary

This study developed dexamethasone acetate-loaded PLGA nanospheres to target liver macrophages for treating inflammatory liver diseases. These nanocarriers show efficient drug delivery and reduced inflammation without significant toxicity.

Keywords:
PLGA nanospheresbiodegradable nanoparticlesglucocorticoidsliver inflammationmacrophages

More Related Videos

Synthesis and Characterization of Placental Chondroitin Sulfate A plCSA-Targeting Lipid-Polymer Nanoparticles
05:55

Synthesis and Characterization of Placental Chondroitin Sulfate A plCSA-Targeting Lipid-Polymer Nanoparticles

Published on: September 18, 2018

8.7K
PLGA Nanoparticles Formed by Single- or Double-emulsion with Vitamin E-TPGS
12:48

PLGA Nanoparticles Formed by Single- or Double-emulsion with Vitamin E-TPGS

Published on: December 27, 2013

65.2K

Related Experiment Videos

Last Updated: Jun 6, 2025

Targeting Drugs to Larval Zebrafish Macrophages by Injecting Drug-Loaded Liposomes
11:31

Targeting Drugs to Larval Zebrafish Macrophages by Injecting Drug-Loaded Liposomes

Published on: February 18, 2020

7.7K
Synthesis and Characterization of Placental Chondroitin Sulfate A plCSA-Targeting Lipid-Polymer Nanoparticles
05:55

Synthesis and Characterization of Placental Chondroitin Sulfate A plCSA-Targeting Lipid-Polymer Nanoparticles

Published on: September 18, 2018

8.7K
PLGA Nanoparticles Formed by Single- or Double-emulsion with Vitamin E-TPGS
12:48

PLGA Nanoparticles Formed by Single- or Double-emulsion with Vitamin E-TPGS

Published on: December 27, 2013

65.2K

Area of Science:

  • Biomedical Engineering
  • Drug Delivery Systems
  • Nanotechnology

Background:

  • Glucocorticoids are effective anti-inflammatory drugs but cause severe side effects.
  • Nanocarriers can reduce glucocorticoid side effects by enabling targeted delivery.
  • Macrophages are key players in inflammation and ideal targets for nanocarrier-based therapies.

Purpose of the Study:

  • To develop dexamethasone acetate (DA)-loaded PLGA nanocarriers for targeted delivery to liver macrophages.
  • To investigate the efficacy of these nanocarriers in reducing inflammation in liver diseases.
  • To assess the safety and biodistribution of the developed nanocarriers.

Main Methods:

  • Dexamethasone acetate (DA)-loaded PLGA nanospheres were synthesized using the nanoprecipitation method.
  • Nanoparticle characterization included size analysis (100-300 nm) and drug loading efficiency (19%).
  • In vitro studies involved macrophage internalization assays and cytokine gene expression analysis; in vivo studies used imaging and flow cytometry for biodistribution.

Main Results:

  • PLGA nanospheres demonstrated sustained DA release over 3 days.
  • Efficient internalization by pro-inflammatory macrophages was observed, with significant down-regulation of pro-inflammatory cytokine genes.
  • In vivo studies confirmed liver macrophage-specific accumulation of nanospheres after intravenous administration.
  • No apparent cytotoxicity was observed.

Conclusions:

  • DA-loaded PLGA nanospheres are a promising drug-delivery system for targeting liver macrophages.
  • This approach offers a potential strategy for localized glucocorticoid release, minimizing systemic side effects.
  • The developed nanocarriers show potential for treating inflammatory liver diseases effectively and safely.